sgBE: a structure-guided design of sgRNA architecture specifies base editing window and enables simultaneous conversion of cytosine and adenosine.
Journal
Genome biology
ISSN: 1474-760X
Titre abrégé: Genome Biol
Pays: England
ID NLM: 100960660
Informations de publication
Date de publication:
28 08 2020
28 08 2020
Historique:
received:
17
01
2020
accepted:
07
08
2020
entrez:
30
8
2020
pubmed:
30
8
2020
medline:
13
7
2021
Statut:
epublish
Résumé
We present a base editing system, in which base editors are attached to different sites of sgRNA scaffold (sgBE). Each independent sgBE has its own specific editing pattern for a given target site. Among tested sgBEs, sgBE-SL4, in which deaminase is attached to the last stem-loop of sgRNA, yields the highest editing efficiency in the window several nucleotides next to the one edited by BE3. sgBE enables the simultaneous editing of adenine and cytosine. Finally, in order to facilitate in vivo base editing, we extend our sgBE system to an AAV-compatible Cas9, SaCas9 (Staphylococcus aureus), and observe robust base editing.
Identifiants
pubmed: 32859244
doi: 10.1186/s13059-020-02137-6
pii: 10.1186/s13059-020-02137-6
pmc: PMC7453718
doi:
Substances chimiques
Nucleotides
0
RNA, Guide
0
Cytosine
8J337D1HZY
CRISPR-Associated Protein 9
EC 3.1.-
Adenine
JAC85A2161
Adenosine
K72T3FS567
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
222Références
ACS Chem Biol. 2016 Mar 18;11(3):681-8
pubmed: 26857072
Mob DNA. 2014 Feb 04;5(1):5
pubmed: 24490831
Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):2984-9
pubmed: 25713377
N Biotechnol. 2017 Jul 25;37(Pt B):194-199
pubmed: 28179151
Nat Biotechnol. 2018 Oct;36(9):843-846
pubmed: 29813047
Nat Biotechnol. 2018 Oct 01;:
pubmed: 30272679
Nat Methods. 2016 Dec;13(12):1029-1035
pubmed: 27723754
Methods. 2017 Feb 1;114:46-53
pubmed: 27474163
Science. 2019 Apr 19;364(6437):289-292
pubmed: 30819928
Traffic. 2008 Aug;9(8):1256-67
pubmed: 18485054
Cell. 2015 Aug 27;162(5):1113-26
pubmed: 26317473
Arterioscler Thromb Vasc Biol. 2017 Sep;37(9):1741-1747
pubmed: 28751571
Nature. 2015 Jan 29;517(7536):583-8
pubmed: 25494202
Nature. 2014 Sep 25;513(7519):569-73
pubmed: 25079318
Sci Adv. 2017 Aug 30;3(8):eaao4774
pubmed: 28875174
Nat Methods. 2016 Dec;13(12):1036-1042
pubmed: 27798611
Nucleic Acids Res. 2014 Jan;42(2):e13
pubmed: 24157833
Mol Ther. 2020 Apr 8;28(4):1177-1189
pubmed: 31991108
Nat Commun. 2017 Nov 9;8(1):1375
pubmed: 29123204
J Biomol Struct Dyn. 1983 Oct;1(2):539-52
pubmed: 6401118
Nat Biotechnol. 2018 Oct;36(9):888-893
pubmed: 29969439
Nat Biotechnol. 2020 Jul;38(7):875-882
pubmed: 31932727
Nat Commun. 2017 Jul 25;8(1):118
pubmed: 28740134
Methods. 2017 Jul 1;123:89-101
pubmed: 28213279
Nature. 2019 May;569(7756):433-437
pubmed: 30995674
Nature. 2017 Nov 23;551(7681):464-471
pubmed: 29160308
J Mol Biol. 1997 Aug 1;270(5):724-38
pubmed: 9245600
Proc Natl Acad Sci U S A. 1995 Apr 25;92(9):4061-5
pubmed: 7732031
Nucleic Acids Res. 2015 Jul 27;43(13):6450-8
pubmed: 26082496
Nat Biotechnol. 2018 Nov;36(10):977-982
pubmed: 30059493
Nature. 2016 Apr 20;533(7603):420-4
pubmed: 27096365
Nat Rev Genet. 2018 Dec;19(12):770-788
pubmed: 30323312
Nat Methods. 2020 Jun;17(6):600-604
pubmed: 32424272
CRISPR J. 2018 Jun;1:239-250
pubmed: 31021262
Nat Biomed Eng. 2020 Jan;4(1):97-110
pubmed: 31937940
Curr Gene Ther. 2005 Jun;5(3):285-97
pubmed: 15975006
Nature. 2008 Nov 6;456(7218):121-4
pubmed: 18849968
J Virol. 2001 Feb;75(4):1824-33
pubmed: 11160681
Nat Biotechnol. 2019 Jun;37(6):626-631
pubmed: 31110355
Nat Biotechnol. 2017 Apr;35(4):371-376
pubmed: 28191901
Mol Plant. 2018 Apr 2;11(4):623-626
pubmed: 29382569
Science. 2019 Apr 19;364(6437):292-295
pubmed: 30819931
Science. 2012 Aug 17;337(6096):816-21
pubmed: 22745249
Yi Chuan. 2019 Sep 20;41(9):777-800
pubmed: 31549678
BioDrugs. 2017 Aug;31(4):317-334
pubmed: 28669112
Biotechnol J. 2014 Nov;9(11):1402-12
pubmed: 25186301
Science. 2013 Feb 15;339(6121):819-23
pubmed: 23287718
Signal Transduct Target Ther. 2019 Sep 20;4:36
pubmed: 31637015