Kinetics of leukemic cells in 3D culture with stromal cells and with arginine deprivation stress.
3D culture
Arginine
Bone marrow stromal cell
Butyrate
Cell cycle
Journal
Journal of bioscience and bioengineering
ISSN: 1347-4421
Titre abrégé: J Biosci Bioeng
Pays: Japan
ID NLM: 100888800
Informations de publication
Date de publication:
Dec 2020
Dec 2020
Historique:
received:
07
04
2020
revised:
08
07
2020
accepted:
28
07
2020
pubmed:
31
8
2020
medline:
19
12
2020
entrez:
31
8
2020
Statut:
ppublish
Résumé
Previously, we established a three-dimensional (3D) bone marrow culture system that maintains normal hematopoiesis, including prolongation of hematopoietic stem cell proliferation and differentiation. To analyze the role of bone marrow stromal cells that compose the microenvironment, the growth of a leukemic cell line (K562) in the 3D condition and with arginine deprivation stress was compared with two-dimensional stromal cell monolayers (2D) and suspension cultures without stromal cells (stroma (-)). Arginine is essential for the proliferation and differentiation of erythrocytes. The proliferation and differentiation of K562 cells cultured in the 3D system were stabilized compared with cells in 2D or stroma (-). Furthermore, the number of K562 cells in the G
Identifiants
pubmed: 32861594
pii: S1389-1723(20)30298-X
doi: 10.1016/j.jbiosc.2020.07.018
pii:
doi:
Substances chimiques
Arginine
94ZLA3W45F
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
650-658Informations de copyright
Copyright © 2020 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.