Acute
Adult
Aged
Calcineurin Inhibitors
/ administration & dosage
Cell Proliferation
/ drug effects
Drug Administration Schedule
Female
Flow Cytometry
/ methods
Graft Rejection
/ immunology
Humans
Immunosuppressive Agents
/ administration & dosage
Inflammation Mediators
/ antagonists & inhibitors
Kidney Transplantation
/ trends
Male
Middle Aged
T-Lymphocytes
/ drug effects
CD25
T-Cell
cyclosporine A
interleukin-2
renal transplantation
tacrolimus.
Journal
Endocrine, metabolic & immune disorders drug targets
ISSN: 2212-3873
Titre abrégé: Endocr Metab Immune Disord Drug Targets
Pays: United Arab Emirates
ID NLM: 101269157
Informations de publication
Date de publication:
2021
2021
Historique:
received:
24
03
2020
revised:
17
07
2020
accepted:
25
07
2020
pubmed:
2
9
2020
medline:
8
1
2022
entrez:
2
9
2020
Statut:
ppublish
Résumé
Calcineurin-inhibitors (CNI) are used in renal transplant patients (RTX) to prevent rejection. CNI mainly suppress T-cell mediated immunity but very little is known about the impact of long-term treatment with CNI on T-cell function. We investigated the immunological effects of long-term CNI intake in RTX patients in comparison to short-term CNI administration in healthy controls (HC). Blood was drawn from 30 RTX patients with long-term CNI treatment. In addition, blood was sampled from HC with short-term CNI treatment (four dosages) before the first and 2 hours after the last CsA intake. T-cells were analyzed for cytokine production, proliferation, and CD25 expression. Short-term CNI reduced T-cell derived IL-2 and IFNγ as well as T-cell proliferation in HC. IFNγ was not suppressed in patients with long-term CNI treatment. IL-2 production, CD25 expression, and T-cell proliferation were enhanced in long-term CNI patients. Suppression of IFNγ/IL-2 and T-cell proliferation is weaker during long-term CNI treatment in patients compared to short-term treatment in healthy subjects. Enhanced CD25 expression may lower the threshold for T-cell activation during long-term CNI treatment.
Sections du résumé
BACKGROUND
BACKGROUND
Calcineurin-inhibitors (CNI) are used in renal transplant patients (RTX) to prevent rejection. CNI mainly suppress T-cell mediated immunity but very little is known about the impact of long-term treatment with CNI on T-cell function.
OBJECTIVE
OBJECTIVE
We investigated the immunological effects of long-term CNI intake in RTX patients in comparison to short-term CNI administration in healthy controls (HC).
METHODS
METHODS
Blood was drawn from 30 RTX patients with long-term CNI treatment. In addition, blood was sampled from HC with short-term CNI treatment (four dosages) before the first and 2 hours after the last CsA intake. T-cells were analyzed for cytokine production, proliferation, and CD25 expression.
RESULTS
RESULTS
Short-term CNI reduced T-cell derived IL-2 and IFNγ as well as T-cell proliferation in HC. IFNγ was not suppressed in patients with long-term CNI treatment. IL-2 production, CD25 expression, and T-cell proliferation were enhanced in long-term CNI patients.
CONCLUSION
CONCLUSIONS
Suppression of IFNγ/IL-2 and T-cell proliferation is weaker during long-term CNI treatment in patients compared to short-term treatment in healthy subjects. Enhanced CD25 expression may lower the threshold for T-cell activation during long-term CNI treatment.
Identifiants
pubmed: 32867664
pii: EMIDDT-EPUB-109576
doi: 10.2174/1871530320999200831161710
doi:
Substances chimiques
Calcineurin Inhibitors
0
Immunosuppressive Agents
0
Inflammation Mediators
0
Types de publication
Comparative Study
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1083-1089Informations de copyright
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