Towards a Multi-Enzyme Capacitive Field-Effect Biosensor by Comparative Study of Drop-Coating and Nano-Spotting Technique.

field-effect EIS biosensor glucose oxidase multi-enzyme immobilization nano-spotting penicillinase urease

Journal

Sensors (Basel, Switzerland)
ISSN: 1424-8220
Titre abrégé: Sensors (Basel)
Pays: Switzerland
ID NLM: 101204366

Informations de publication

Date de publication:
31 Aug 2020
Historique:
received: 23 07 2020
revised: 24 08 2020
accepted: 29 08 2020
entrez: 4 9 2020
pubmed: 4 9 2020
medline: 20 3 2021
Statut: epublish

Résumé

Multi-enzyme immobilization onto a capacitive field-effect biosensor by nano-spotting technique is presented. The nano-spotting technique allows to immobilize different enzymes simultaneously on the sensor surface with high spatial resolution without additional photolithographical patterning. The amount of applied enzymatic cocktail on the sensor surface can be tailored. Capacitive electrolyte-insulator-semiconductor (EIS) field-effect sensors with Ta

Identifiants

pubmed: 32878210
pii: s20174924
doi: 10.3390/s20174924
pmc: PMC7506612
pii:
doi:

Substances chimiques

Enzymes, Immobilized 0
Urease EC 3.5.1.5

Types de publication

Letter

Langues

eng

Sous-ensembles de citation

IM

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Auteurs

Denise Molinnus (D)

Institute of Nano- and Biotechnologies (INB), FH Aachen, Campus Jülich, 52428 Jülich, Germany.

Stefan Beging (S)

Institute of Nano- and Biotechnologies (INB), FH Aachen, Campus Jülich, 52428 Jülich, Germany.

Carsten Lowis (C)

Institute of Nano- and Biotechnologies (INB), FH Aachen, Campus Jülich, 52428 Jülich, Germany.

Michael J Schöning (MJ)

Institute of Nano- and Biotechnologies (INB), FH Aachen, Campus Jülich, 52428 Jülich, Germany.
Institute of Biological Information Processing (IBI-3), Research Center Jülich, 52425 Jülich, Germany.

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Classifications MeSH