Heterozygous retinoblastoma gene mutation compromises in vitro osteogenesis of adipose mesenchymal stem cells - a temporal gene expression study.
Adipocytes
/ metabolism
Adipose Tissue
/ metabolism
Adult
Bone Neoplasms
/ genetics
Cell Differentiation
Cell Transformation, Neoplastic
Chondrocytes
/ metabolism
Core Binding Factor Alpha 1 Subunit
/ genetics
DNA-Binding Proteins
/ genetics
Estrogen Receptor alpha
/ genetics
Female
Gene Expression Profiling
Gene Expression Regulation, Neoplastic
Heterozygote
Humans
Male
Mesenchymal Stem Cells
/ metabolism
Mutation
Orbit
/ metabolism
Osteogenesis
/ genetics
Osteosarcoma
/ genetics
Primary Cell Culture
Receptor, IGF Type 1
/ genetics
Receptors, Androgen
/ genetics
Retinal Neoplasms
/ genetics
Retinoblastoma Binding Proteins
/ genetics
Signal Transduction
Sp7 Transcription Factor
/ genetics
Ubiquitin-Protein Ligases
/ genetics
Hormone receptor
Mesenchymal stem cells
Osteogenesis
Osteosarcoma
Retinoblastoma
Tumor suppressor
Journal
Experimental cell research
ISSN: 1090-2422
Titre abrégé: Exp Cell Res
Pays: United States
ID NLM: 0373226
Informations de publication
Date de publication:
01 11 2020
01 11 2020
Historique:
received:
23
04
2020
revised:
28
08
2020
accepted:
30
08
2020
pubmed:
6
9
2020
medline:
12
3
2021
entrez:
5
9
2020
Statut:
ppublish
Résumé
Osteosarcoma (OS) is a bone malignancy affecting children and adolescents. Retinoblastoma (RB) patients with germline RB1 mutations are susceptible to osteosarcoma in the second decade of their life. Several studies, particularly in mice, have revealed a role for RB1 in osteogenesis. Since, there is species specific difference attributed in retinoblastoma tumorigenesis between mice and human, we assumed, it is worthwhile exploring the role of RB1 in osteogenesis and thus onset of osteosarcoma. In this study, we analyzed the temporal gene expression of the osteogenic markers, tumor suppressor genes and hormone receptors associated with growth spurt during in vitro osteogenesis of mesenchymal stem cells derived from orbital adipose tissue of germline RB patients and compared it with those with wild type RB1 gene. Mesenchymal stem cells with the heterozygous RB1 mutation showed reduced expression of RB1 and other tumor suppressor genes and showed deregulation of osteogenic markers which could be an initial step for the onset of osteosarcoma.
Identifiants
pubmed: 32890459
pii: S0014-4827(20)30512-7
doi: 10.1016/j.yexcr.2020.112263
pii:
doi:
Substances chimiques
Core Binding Factor Alpha 1 Subunit
0
DNA-Binding Proteins
0
ESR1 protein, human
0
Estrogen Receptor alpha
0
IGF1R protein, human
0
RB1 protein, human
0
RUNX2 protein, human
0
Receptors, Androgen
0
Retinoblastoma Binding Proteins
0
Sp7 Transcription Factor
0
SP7 protein, human
0
zinc finger protein 521, human
0
Ubiquitin-Protein Ligases
EC 2.3.2.27
Receptor, IGF Type 1
EC 2.7.10.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
112263Informations de copyright
Copyright © 2020 Elsevier Inc. All rights reserved.