CRISPR/Cas12a-based dual amplified biosensing system for sensitive and rapid detection of polynucleotide kinase/phosphatase.

Biosensing Techniques CRISPR-Cas Systems / genetics Clustered Regularly Interspaced Short Palindromic Repeats Phosphoric Monoester Hydrolases Polynucleotide 5'-Hydroxyl-Kinase / genetics
Biosensing CRISPR/Cas12a Nicking enzyme Polynucleotide kinase/phosphatase (PNKP) Strand displacement amplification

Journal

Biosensors & bioelectronics
ISSN: 1873-4235
Titre abrégé: Biosens Bioelectron
Pays: England
ID NLM: 9001289

Informations de publication

Date de publication:
15 Nov 2020
Historique:
received: 12 07 2020
revised: 14 08 2020
accepted: 24 08 2020
pubmed: 6 9 2020
medline: 15 5 2021
entrez: 5 9 2020
Statut: ppublish

Résumé

We reported a CRISPR/Cas-based dual amplified sensing strategy for rapid, sensitive and selective detection of polynucleotide kinase/phosphatase (PNKP), a DNA damage repair-related biological enzyme. In this strategy, a PNKP-triggered nicking enzyme-mediated strand displacement amplification reaction was introduced to enrich the activator DNA strands for CRISPR/Cas. Such an isothermal DNA amplification step, together with subsequent activated CRISPR/Cas-catalyzed cleavage of fluorescent-labeled short-stranded DNA probes, enable synergetic signal amplification for sensitive PNKP detection. The proposed strategy showed a wide linear detection range (more than 3 orders of magnitude ranging from 1× 10

Identifiants

DOI: 10.1016/j.bios.2020.112556 PMID: 32890931
pubmed: 32890931
pii: S0956-5663(20)30548-0
doi: 10.1016/j.bios.2020.112556
pii:
doi:

Substances chimiques

Polynucleotide 5'-Hydroxyl-Kinase EC 2.7.1.78
Phosphoric Monoester Hydrolases EC 3.1.3.2

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

112556

Informations de copyright

Copyright © 2020 Elsevier B.V. All rights reserved.

Auteurs

Dong-Xia Wang (DX)

State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Biosensing and Molecular Recognition, Research Centre for Analytical Sciences, College of Chemistry, Nankai University, Tianjin, 300071, PR China.

Jing Wang (J)

State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Biosensing and Molecular Recognition, Research Centre for Analytical Sciences, College of Chemistry, Nankai University, Tianjin, 300071, PR China.

Yi-Chen Du (YC)

State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Biosensing and Molecular Recognition, Research Centre for Analytical Sciences, College of Chemistry, Nankai University, Tianjin, 300071, PR China.

Jia-Yi Ma (JY)

State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Biosensing and Molecular Recognition, Research Centre for Analytical Sciences, College of Chemistry, Nankai University, Tianjin, 300071, PR China.

Si-Yuan Wang (SY)

State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Biosensing and Molecular Recognition, Research Centre for Analytical Sciences, College of Chemistry, Nankai University, Tianjin, 300071, PR China.

An-Na Tang (AN)

State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Biosensing and Molecular Recognition, Research Centre for Analytical Sciences, College of Chemistry, Nankai University, Tianjin, 300071, PR China.

De-Ming Kong (DM)

State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Biosensing and Molecular Recognition, Research Centre for Analytical Sciences, College of Chemistry, Nankai University, Tianjin, 300071, PR China. Electronic address: kongdem@nankai.edu.cn.

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Classifications MeSH

questionsmedicales.fr › Techniques d'investigation › Techniques de sonde moléculaire › Techniques de biocapteur › CRISPR/Cas12a-based dual amplified biosensing...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Épigenèse génétique › Extinction de l'expression des gènes › Systèmes CRISPR-Cas › CRISPR/Cas12a-based dual amplified biosensing...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Composants de génome › Séquences répétées en tandem › Clustered regularly interspaced short palindromic repeats › CRISPR/Cas12a-based dual amplified biosensing...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Esterases › Phosphoric monoester hydrolases › CRISPR/Cas12a-based dual amplified biosensing...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Transferases › Phosphotransferases › Phosphotransferases (Alcohol Group Acceptor) › Polynucleotide 5'-hydroxyl-kinase › CRISPR/Cas12a-based dual amplified biosensing...