Knock-down of δ-aminolevulinic acid dehydratase via virus-induced gene silencing alters the microRNA biogenesis and causes stress-related reactions in citrus plants.
Citrus
H(2)O(2)
Virus-induced gene silencing
miR167
miR393
miRNA
δ-Aminolevulinic acid dehydratase
Journal
Plant science : an international journal of experimental plant biology
ISSN: 1873-2259
Titre abrégé: Plant Sci
Pays: Ireland
ID NLM: 9882015
Informations de publication
Date de publication:
Oct 2020
Oct 2020
Historique:
received:
31
05
2020
revised:
21
07
2020
accepted:
23
07
2020
entrez:
9
9
2020
pubmed:
10
9
2020
medline:
2
3
2021
Statut:
ppublish
Résumé
The δ-aminolevulinic acid (δ-ALA) is an intermediate in the biosynthetic pathway of tetrapyrroles. Tetrapyrroles play vital roles in many biological processes such as photosynthesis, respiration, and light-sensing. ALA-dehydratase (ALAD) combines two molecules of δ-ALA to form porphobilinogen. In citrus, the silencing of ALAD caused discrete yellow spots and necrosis in leaves and stems. Additionally, it caused rapid death in developing new shoots. Herein, we hypothesize that the accumulation of δ-ALA results in severe stress and reduced meristem development. For that reason, we investigated the dynamic changes in the expression profiles of 23 microRNA (miRNA) identified through small RNA sequencing, from CTV-tALAD plants in comparison with healthy C. macrophylla and C. macrophylla infiltrated with CTV-wt. Furthermore, we reported the effect of ALAD silencing on the total phenolics, H
Identifiants
pubmed: 32900450
pii: S0168-9452(20)30228-4
doi: 10.1016/j.plantsci.2020.110622
pii:
doi:
Substances chimiques
MicroRNAs
0
Phenols
0
RNA, Plant
0
Reactive Oxygen Species
0
Hydrogen Peroxide
BBX060AN9V
Porphobilinogen Synthase
EC 4.2.1.24
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
110622Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.