The Arabidopsis PHD-finger protein EDM2 has multiple roles in balancing NLR immune receptor gene expression.
Arabidopsis
Arabidopsis Proteins
/ genetics
Epigenesis, Genetic
Gene Expression
Gene Expression Regulation, Plant
Genes, Plant
Jumonji Domain-Containing Histone Demethylases
/ genetics
NLR Proteins
/ biosynthesis
PHD Zinc Fingers
Plants, Genetically Modified
Protein Domains
RNA-Binding Proteins
/ genetics
Receptors, Immunologic
/ genetics
Transcription Factors
/ genetics
Journal
PLoS genetics
ISSN: 1553-7404
Titre abrégé: PLoS Genet
Pays: United States
ID NLM: 101239074
Informations de publication
Date de publication:
09 2020
09 2020
Historique:
received:
26
09
2019
accepted:
14
07
2020
revised:
01
10
2020
pubmed:
15
9
2020
medline:
18
11
2020
entrez:
14
9
2020
Statut:
epublish
Résumé
Plant NLR-type receptors serve as sensitive triggers of host immunity. Their expression has to be well-balanced, due to their interference with various cellular processes and dose-dependency of their defense-inducing activity. A genetic "arms race" with fast-evolving pathogenic microbes requires plants to constantly innovate their NLR repertoires. We previously showed that insertion of the COPIA-R7 retrotransposon into RPP7 co-opted the epigenetic transposon silencing signal H3K9me2 to a new function promoting expression of this Arabidopsis thaliana NLR gene. Recruitment of the histone binding protein EDM2 to COPIA-R7-associated H3K9me2 is required for optimal expression of RPP7. By profiling of genome-wide effects of EDM2, we now uncovered additional examples illustrating effects of transposons on NLR gene expression, strongly suggesting that these mobile elements can play critical roles in the rapid evolution of plant NLR genes by providing the "raw material" for gene expression mechanisms. We further found EDM2 to have a global role in NLR expression control. Besides serving as a positive regulator of RPP7 and a small number of other NLR genes, EDM2 acts as a suppressor of a multitude of additional NLR genes. We speculate that the dual functionality of EDM2 in NLR expression control arose from the need to compensate for fitness penalties caused by high expression of some NLR genes by suppression of others. Moreover, we are providing new insights into functional relationships of EDM2 with its interaction partner, the RNA binding protein EDM3/AIPP1, and its target gene IBM1, encoding an H3K9-demethylase.
Identifiants
pubmed: 32925902
doi: 10.1371/journal.pgen.1008993
pii: PGENETICS-D-19-01617
pmc: PMC7529245
doi:
Substances chimiques
Arabidopsis Proteins
0
EDM2 protein, Arabidopsis
0
NLR Proteins
0
RNA-Binding Proteins
0
Receptors, Immunologic
0
Transcription Factors
0
IBM1 protein, Arabidopsis
EC 1.14.11.-
Jumonji Domain-Containing Histone Demethylases
EC 1.14.11.-
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1008993Subventions
Organisme : NIAID NIH HHS
ID : R01 AI106775
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI136511
Pays : United States
Organisme : NIH HHS
ID : S10 OD010669
Pays : United States
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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