Novel features of centriole polarity and cartwheel stacking revealed by cryo-tomography.
Teranympha
Trichonympha
SAS-6
cartwheel
centriole
microtubules
Journal
The EMBO journal
ISSN: 1460-2075
Titre abrégé: EMBO J
Pays: England
ID NLM: 8208664
Informations de publication
Date de publication:
16 11 2020
16 11 2020
Historique:
received:
15
07
2020
revised:
11
08
2020
accepted:
21
08
2020
pubmed:
22
9
2020
medline:
15
4
2021
entrez:
21
9
2020
Statut:
ppublish
Résumé
Centrioles are polarized microtubule-based organelles that seed the formation of cilia, and which assemble from a cartwheel containing stacked ring oligomers of SAS-6 proteins. A cryo-tomography map of centrioles from the termite flagellate Trichonympha spp. was obtained previously, but higher resolution analysis is likely to reveal novel features. Using sub-tomogram averaging (STA) in T. spp. and Trichonympha agilis, we delineate the architecture of centriolar microtubules, pinhead, and A-C linker. Moreover, we report ~25 Å resolution maps of the central cartwheel, revealing notably polarized cartwheel inner densities (CID). Furthermore, STA of centrioles from the distant flagellate Teranympha mirabilis uncovers similar cartwheel architecture and a distinct filamentous CID. Fitting the CrSAS-6 crystal structure into the flagellate maps and analyzing cartwheels generated in vitro indicate that SAS-6 rings can directly stack onto one another in two alternating configurations: with a slight rotational offset and in register. Overall, improved STA maps in three flagellates enabled us to unravel novel architectural features, including of centriole polarity and cartwheel stacking, thus setting the stage for an accelerated elucidation of underlying assembly mechanisms.
Identifiants
pubmed: 32954505
doi: 10.15252/embj.2020106249
pmc: PMC7667878
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e106249Subventions
Organisme : EC|FP7|FP7 Ideas: European Research Council (FP7 Ideas)
ID : 340227
Organisme : Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (FNS)
ID : PP00P3_157517
Informations de copyright
© 2020 The Authors. Published under the terms of the CC BY 4.0 license.
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