A small nucleolar RNA, SNORD126, promotes adipogenesis in cells and rats by activating the PI3K-AKT pathway.
3T3-L1 Cells
Adipocytes
/ cytology
Adipogenesis
/ genetics
Adipose Tissue
/ cytology
Animals
Cell Differentiation
/ genetics
Diet, High-Fat
G1 Phase
Gene Expression Regulation
Humans
Male
Mice
Obesity
/ genetics
Phosphatidylinositol 3-Kinases
/ metabolism
Proto-Oncogene Proteins c-akt
/ metabolism
RNA, Small Nucleolar
/ metabolism
Rats, Sprague-Dawley
S Phase
Signal Transduction
Stem Cells
/ cytology
Akt
SNORD126
adipogenesis
p70S6K
small nucleolar RNA
Journal
Journal of cellular physiology
ISSN: 1097-4652
Titre abrégé: J Cell Physiol
Pays: United States
ID NLM: 0050222
Informations de publication
Date de publication:
04 2021
04 2021
Historique:
received:
10
03
2020
revised:
04
09
2020
accepted:
07
09
2020
pubmed:
23
9
2020
medline:
22
9
2021
entrez:
22
9
2020
Statut:
ppublish
Résumé
Small nucleolar RNA (snoRNA) plays important role in various histogenesis. Whether snoRNA plays a role in adipogenesis is unknown. SNORD126 is a C/D box snoRNA. We previously demonstrated that SNORD126 promoted hepatocellular carcinoma cell growth by activating the phosphoinositide 3-kinase-protein kinase B (Akt) pathway through upregulating fibroblast growth factor receptor 2 expression. In the present study, we found that the expression of SNORD126 was downregulated in the obesity-related tissues in high-fat diet-fed rats. Overexpression of SNORD126 in 3T3-L1 cells promoted adipocytes differentiation. SNORD126 significantly increased the expression of CCAAT/enhancer-binding protein α, fatty acid-binding protein 4, peroxisome proliferative-activated receptor-γ, and the phosphorylation of Akt and p70S6K. Overexpression of SNORD126 in human adipose-derived stem cells stimulated adipogenesis and increased phosphorylation of Akt. Meanwhile, SNORD126 increased the messenger RNA and protein levels of cyclin D1 and cyclin-dependent kinase 2, which promoted mitotic clonal expansion progression during the early stage of 3T3-L1 cell differentiation. We further found that SNORD126 accelerated the growth of the groin fat pad and increased phosphorylation of Akt and p70S6K in rats. Overall, our results suggested that SNORD126 promoted adipocyte differentiation through increasing phosphorylation of Akt and p70S6K both in vitro and in vivo.
Substances chimiques
RNA, Small Nucleolar
0
Proto-Oncogene Proteins c-akt
EC 2.7.11.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3001-3014Informations de copyright
© 2020 Wiley Periodicals LLC.
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