RovC - a novel type of hexameric transcriptional activator promoting type VI secretion gene expression.
Journal
PLoS pathogens
ISSN: 1553-7374
Titre abrégé: PLoS Pathog
Pays: United States
ID NLM: 101238921
Informations de publication
Date de publication:
09 2020
09 2020
Historique:
received:
15
04
2020
accepted:
01
08
2020
revised:
05
10
2020
pubmed:
24
9
2020
medline:
22
10
2020
entrez:
23
9
2020
Statut:
epublish
Résumé
Type VI secretion systems (T6SSs) are complex macromolecular injection machines which are widespread in Gram-negative bacteria. They are involved in host-cell interactions and pathogenesis, required to eliminate competing bacteria, or are important for the adaptation to environmental stress conditions. Here we identified regulatory elements controlling the T6SS4 of Yersinia pseudotuberculosis and found a novel type of hexameric transcription factor, RovC. RovC directly interacts with the T6SS4 promoter region and activates T6SS4 transcription alone or in cooperation with the LysR-type regulator RovM. A higher complexity of regulation was achieved by the nutrient-responsive global regulator CsrA, which controls rovC expression on the transcriptional and post-transcriptional level. In summary, our work unveils a central mechanism in which RovC, a novel key activator, orchestrates the expression of the T6SS weapons together with a global regulator to deploy the system in response to the availability of nutrients in the species' native environment.
Identifiants
pubmed: 32966346
doi: 10.1371/journal.ppat.1008552
pii: PPATHOGENS-D-20-00764
pmc: PMC7535981
doi:
Substances chimiques
Bacterial Proteins
0
Type VI Secretion Systems
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1008552Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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