Genomic analysis of cardiac surgery-associated Mycobacterium chimaera infections in Italy.
Cardiac Surgical Procedures
/ adverse effects
Cross Infection
/ genetics
Disease Outbreaks
Equipment Contamination
Female
Genomics
Humans
Italy
/ epidemiology
Male
Mycobacterium
/ isolation & purification
Mycobacterium Infections
/ epidemiology
Mycobacterium Infections, Nontuberculous
/ epidemiology
Nontuberculous Mycobacteria
/ genetics
Polymorphism, Single Nucleotide
/ genetics
Water Microbiology
Whole Genome Sequencing
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2020
2020
Historique:
received:
06
04
2020
accepted:
02
09
2020
entrez:
25
9
2020
pubmed:
26
9
2020
medline:
5
11
2020
Statut:
epublish
Résumé
One hundred and twenty-two Mycobacterium chimaera strains isolated in Italy from cardiac surgery-related patients, cardiac surgery-unrelated patients and from heater-cooler units, were submitted to whole-genome sequencing and to subsequent SNP analysis. All but one strains isolated from cardiac surgery-related patients belonged to Subgroup 1.1 (19/23) or Subgroup 1.8 (3/23). Only 28 out of 79 strains isolated from heater-cooler units belonged to groupings other than 1.1 and 1.8. The strains isolated from cardiac surgery-unrelated patients were instead distributed across the phylogenetic tree. Our data, the first on isolates from Italy, are in agreement with a recent large genomic study suggesting a common source, represented by strains belonging to Subgroups 1.1 and 1.8, of cardiac surgery-related Mycobacterium chimaera infections. The strains belonging to groupings other than 1.1 and 1.8 isolated from heather-cooler units evidently resulted from contaminations at hospital level and had no share in the Mycobacterium chimaera outbreak. One Mycobacterium chimaera strain investigated in this study proved distant from every previously known Mycobacterium chimaera Groups (1, 2, 3 and 4) and we propose to assign to a novel group, named "Group 5".
Identifiants
pubmed: 32976495
doi: 10.1371/journal.pone.0239273
pii: PONE-D-20-09309
pmc: PMC7518601
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0239273Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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