Regeneration of Burnt Bridges on a DNA Catenane Walker.
DNA catenane walkers
DNA nanotechnology
biohybrids
molecular machines
Journal
Angewandte Chemie (International ed. in English)
ISSN: 1521-3773
Titre abrégé: Angew Chem Int Ed Engl
Pays: Germany
ID NLM: 0370543
Informations de publication
Date de publication:
14 09 2020
14 09 2020
Historique:
received:
26
03
2020
entrez:
30
9
2020
pubmed:
1
10
2020
medline:
23
3
2021
Statut:
ppublish
Résumé
DNA walkers are molecular machines that can move with high precision onthe nanoscale due to their structural and functional programmability. Despite recent advances in the field that allow exploring different energy sources, stimuli, and mechanisms of action for these nanomachines, the continuous operation and reusability of DNA walkers remains challenging because in most cases the steps, once taken by the walker, cannot be taken again. Herein we report the path regeneration of a burnt-bridges DNA catenane walker using RNase A. This walker uses a T7RNA polymerase that produces long RNA transcripts to hybridize to the path and move forward while the RNA remains hybridized to the path and blocks it for an additional walking cycle. We show that RNA degradation triggered by RNase A restores the path and returns the walker to the initial position. RNase inhibition restarts the function of the walker.
Identifiants
pubmed: 32997429
doi: 10.1002/anie.202004447
pmc: PMC7540537
doi:
Substances chimiques
DNA, Catenated
0
Viral Proteins
0
RNA
63231-63-0
bacteriophage T7 RNA polymerase
EC 2.7.7.-
DNA-Directed RNA Polymerases
EC 2.7.7.6
Ribonuclease, Pancreatic
EC 3.1.27.5
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
16366-16370Informations de copyright
© 2020 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA.
Références
Nat Nanotechnol. 2014 Jan;9(1):39-43
pubmed: 24317284
Curr Opin Biotechnol. 2015 Aug;34:56-64
pubmed: 25498478
Phys Rev Lett. 2012 Dec 7;109(23):238104
pubmed: 23368271
Angew Chem Int Ed Engl. 2020 Sep 14;59(38):16366-16370
pubmed: 32997429
Nano Lett. 2017 Sep 13;17(9):5368-5374
pubmed: 28750513
J Am Chem Soc. 2016 Apr 27;138(16):5172-85
pubmed: 27019201
Chemphyschem. 2009 Oct 19;10(15):2593-7
pubmed: 19739195
Nature. 2010 May 13;465(7295):206-10
pubmed: 20463735
Nanoscale. 2018 May 17;10(19):9199-9211
pubmed: 29726566
ACS Synth Biol. 2016 Aug 19;5(8):878-84
pubmed: 27114350
Bioengineered. 2018 Jan 1;9(1):90-97
pubmed: 28662363
Nat Nanotechnol. 2010 Nov;5(11):778-82
pubmed: 20935654
Chem Rev. 2020 Jan 8;120(1):288-309
pubmed: 31509383
Angew Chem Int Ed Engl. 2005 Jul 11;44(28):4358-61
pubmed: 15959864
Chem Rev. 2019 May 22;119(10):6326-6369
pubmed: 30714375
Angew Chem Int Ed Engl. 2005 Jul 11;44(28):4355-8
pubmed: 15945114
Science. 2007 Jul 20;317(5836):333-6
pubmed: 17641191
Chem Rev. 2015 Sep 23;115(18):10081-206
pubmed: 26346838
Nanoscale. 2019 May 16;11(19):9240-9263
pubmed: 31062798
Chembiochem. 2016 Jun 16;17(12):1138-41
pubmed: 27059426
Science. 2017 Sep 15;357(6356):
pubmed: 28912216
Nat Commun. 2019 Dec 11;10(1):5655
pubmed: 31827087
Nature. 2003 Apr 17;422(6933):759-65
pubmed: 12700770
Nature. 2010 May 13;465(7295):202-5
pubmed: 20463734
Nano Lett. 2011 Jan 12;11(1):304-9
pubmed: 21166467
Chem Asian J. 2019 Jul 15;14(14):2406-2416
pubmed: 31042016
Curr Opin Biotechnol. 2017 Dec;48:159-167
pubmed: 28505598
Nature. 1999 Sep 9;401(6749):152-5
pubmed: 10490022
Chem Rev. 2019 May 22;119(10):6370-6383
pubmed: 30865429
ACS Nano. 2016 Jun 28;10(6):5882-90
pubmed: 27294366
ACS Nano. 2017 Apr 25;11(4):4002-4008
pubmed: 28402651
Angew Chem Int Ed Engl. 2004 Sep 20;43(37):4906-11
pubmed: 15372637
Angew Chem Int Ed Engl. 2014 Oct 13;53(42):11102-4
pubmed: 25257242
Chem Rev. 2005 Apr;105(4):1377-400
pubmed: 15826015
Chem Soc Rev. 2017 May 9;46(9):2592-2621
pubmed: 28426052
Annu Rev Biochem. 1969;38:677-732
pubmed: 4308605
Angew Chem Int Ed Engl. 2019 May 20;58(21):6948-6951
pubmed: 30897257
Chemphyschem. 2008 Aug 4;9(11):1503-9
pubmed: 18618534
Nat Nanotechnol. 2018 Jun;13(6):496-503
pubmed: 29632399
Nat Protoc. 2019 Oct;14(10):2818-2855
pubmed: 31511665
Nature. 2016 Jun 08;534(7606):235-40
pubmed: 27279219
J Phys Condens Matter. 2013 Nov 20;25(46):463101
pubmed: 24100357
Nanotechnol Sci Appl. 2019 Oct 15;12:25-46
pubmed: 31686793
Angew Chem Int Ed Engl. 2012 Mar 5;51(10):2457-60
pubmed: 22298502
Angew Chem Int Ed Engl. 2017 Sep 4;56(37):11094-11125
pubmed: 28815900
Nature. 2011 Nov 09;479(7372):208-11
pubmed: 22071765
Chem Commun (Camb). 2014 Jun 11;50(46):6091-3
pubmed: 24777123
J Biol Chem. 2003 Jun 6;278(23):20934-8
pubmed: 12649287