Chicken Astrovirus (CAstV) Molecular Studies Reveal Evidence of Multiple Past Recombination Events in Sequences Originated from Clinical Samples of White Chick Syndrome (WCS) in Western Canada.


Journal

Viruses
ISSN: 1999-4915
Titre abrégé: Viruses
Pays: Switzerland
ID NLM: 101509722

Informations de publication

Date de publication:
28 09 2020
Historique:
received: 12 09 2020
revised: 22 09 2020
accepted: 25 09 2020
entrez: 1 10 2020
pubmed: 2 10 2020
medline: 9 3 2021
Statut: epublish

Résumé

In this study, we aimed to molecularly characterize 14 whole genome sequences of chicken astrovirus (CAstV) isolated from samples obtained from white chick syndrome (WCS) outbreaks in Western Canada during the period of 2014-2019. Genome sequence comparisons showed all these sequences correspond to the novel Biv group from which no confirmed representatives were published in GenBank. Molecular recombination analyses using recombination detection software (i.e., RDP5 and SimPlot) and phylogenetic analyses suggest multiple past recombination events in open reading frame (ORF)1a, ORF1b, and ORF2. Our findings suggest that recombination events and the accumulation of point mutations may have contributed to the substantial genetic variation observed in CAstV and evidenced by the current seven antigenic sub-clusters hitherto described. This is the first paper that describes recombination events in CAstV following analysis of complete CAstV sequences originated in Canada.

Identifiants

pubmed: 32998356
pii: v12101096
doi: 10.3390/v12101096
pmc: PMC7600043
pii:
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

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Auteurs

Victor Palomino-Tapia (V)

Department of Ecosystem and Public Health, Faculty of Veterinary Medicine, University of Calgary, Health Research Innovation Center 2C53, 3330 Hospital Drive NW, Calgary, AB T2N 4N1, Canada.

Darko Mitevski (D)

Poultry Health Services, 1-4 East Lake Ave NE, Airdrie, AB T4A 2G8, Canada.

Tom Inglis (T)

The Institute of Applied Poultry Technologies, 201-151 East Lake Blvd, Airdrie, AB T4A 2G1, Canada.

Frank van der Meer (F)

Department of Ecosystem and Public Health, Faculty of Veterinary Medicine, University of Calgary, Health Research Innovation Center 2C53, 3330 Hospital Drive NW, Calgary, AB T2N 4N1, Canada.

Emily Martin (E)

Animal Health Laboratory, University of Guelph, Guelph, ON N1H 6R8, Canada.

Marina Brash (M)

Animal Health Laboratory, University of Guelph, Guelph, ON N1H 6R8, Canada.

Chantale Provost (C)

Swine and poultry infectious diseases research center (CRIPA Fonds de Recherche du Quebec), Molecular Diagnostic Laboratory, Faculty of veterinary medicine, University of Montreal, 3200 Sicotte, Saint-Hyacinthe, QC J2S 2M2, Canada.

Carl A Gagnon (CA)

Swine and poultry infectious diseases research center (CRIPA Fonds de Recherche du Quebec), Molecular Diagnostic Laboratory, Faculty of veterinary medicine, University of Montreal, 3200 Sicotte, Saint-Hyacinthe, QC J2S 2M2, Canada.

Mohamed Faizal Abdul-Careem (MF)

Department of Ecosystem and Public Health, Faculty of Veterinary Medicine, University of Calgary, Health Research Innovation Center 2C53, 3330 Hospital Drive NW, Calgary, AB T2N 4N1, Canada.

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