Next-generation sequencing in the diagnosis of viral encephalitis: sensitivity and clinical limitations.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
30 09 2020
30 09 2020
Historique:
received:
16
03
2020
accepted:
07
09
2020
entrez:
1
10
2020
pubmed:
2
10
2020
medline:
29
12
2020
Statut:
epublish
Résumé
Identification of pathogens causing viral encephalitis remains challenging, and in over 50% of cases the etiologic factor remains undetermined. Next-generation sequencing (NGS) based metagenomics has been successfully used to detect novel and rare infections, but its value for routine diagnosis of encephalitis remains unclear. The aim of the present study was to determine the sensitivity of shotgun metagenomic sequencing protocols, which include preamplification, and testing it against cerebrospinal fluid (CSF) samples from encephalitis patients. For sensitivity testing HIV and HBV positive sera were serially diluted in CSF from an uninfected patient. NGS repeatedly detected HIV and HBV sequences present at concentrations from 10
Identifiants
pubmed: 32999423
doi: 10.1038/s41598-020-73156-3
pii: 10.1038/s41598-020-73156-3
pmc: PMC7528011
doi:
Substances chimiques
DNA, Viral
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
16173Références
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