The ex planta signal activity of a Medicago ribosomal uL2 protein suggests a moonlighting role in controlling secondary rhizobial infection.
Coinfection
/ prevention & control
Ethylenes
/ metabolism
Gene Expression Regulation, Plant
Genes, Plant
Medicago truncatula
/ genetics
Plant Immunity
/ genetics
Plant Proteins
/ genetics
Plant Root Nodulation
/ physiology
Plant Roots
/ metabolism
Ribosomal Proteins
/ genetics
Root Nodules, Plant
/ metabolism
Signal Transduction
Sinorhizobium meliloti
/ metabolism
Symbiosis
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2020
2020
Historique:
received:
27
02
2020
accepted:
15
06
2020
entrez:
1
10
2020
pubmed:
2
10
2020
medline:
13
11
2020
Statut:
epublish
Résumé
We recently described a regulatory loop, which we termed autoregulation of infection (AOI), by which Sinorhizobium meliloti, a Medicago endosymbiont, downregulates the root susceptibility to secondary infection events via ethylene. AOI is initially triggered by so-far unidentified Medicago nodule signals named signal 1 and signal 1' whose transduction in bacteroids requires the S. meliloti outer-membrane-associated NsrA receptor protein and the cognate inner-membrane-associated adenylate cyclases, CyaK and CyaD1/D2, respectively. Here, we report on advances in signal 1 identification. Signal 1 activity is widespread as we robustly detected it in Medicago nodule extracts as well as in yeast and bacteria cell extracts. Biochemical analyses indicated a peptidic nature for signal 1 and, together with proteomic analyses, a universally conserved Medicago ribosomal protein of the uL2 family was identified as a candidate signal 1. Specifically, MtRPuL2A (MtrunA17Chr7g0247311) displays a strong signal activity that requires S. meliloti NsrA and CyaK, as endogenous signal 1. We have shown that MtRPuL2A is active in signaling only in a non-ribosomal form. A Medicago truncatula mutant in the major symbiotic transcriptional regulator MtNF-YA1 lacked most signal 1 activity, suggesting that signal 1 is under developmental control. Altogether, our results point to the MtRPuL2A ribosomal protein as the candidate for signal 1. Based on the Mtnf-ya1 mutant, we suggest a link between root infectiveness and nodule development. We discuss our findings in the context of ribosomal protein moonlighting.
Identifiants
pubmed: 33002000
doi: 10.1371/journal.pone.0235446
pii: PONE-D-20-05716
pmc: PMC7529298
doi:
Substances chimiques
Ethylenes
0
Plant Proteins
0
Ribosomal Proteins
0
ethylene
91GW059KN7
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0235446Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist
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