Validation of a Combined Transcriptome and T Cell Receptor Alpha/Beta (TRA/TRB) Repertoire Assay at the Single Cell Level for Paucicellular Samples.
cDNA
combined transcriptome and T cell receptor repertoire assay
low cell number
single T cell receptor repertoire analysis
single cell transcriptomics
Journal
Frontiers in immunology
ISSN: 1664-3224
Titre abrégé: Front Immunol
Pays: Switzerland
ID NLM: 101560960
Informations de publication
Date de publication:
2020
2020
Historique:
received:
12
05
2020
accepted:
23
07
2020
entrez:
5
10
2020
pubmed:
6
10
2020
medline:
21
5
2021
Statut:
epublish
Résumé
Transcriptomics can be combined with TRA and TRB clonotype analysis at the single cell level. The aim of this study was to validate this approach on the ICELL8 Single-Cell system and to evaluate its usefulness to analyse clinical paucicellular samples. For this purpose, we carefully selected T cell lines with defined TRA/TRB clonotypes as well as clinical samples enriched for CD3
Identifiants
pubmed: 33013853
doi: 10.3389/fimmu.2020.01999
pmc: PMC7500136
doi:
Substances chimiques
Receptors, Antigen, T-Cell, alpha-beta
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1999Informations de copyright
Copyright © 2020 Litjens, Langerak, van der List, Klepper, de Bie, Azmani, den Dekker, Brouwer, Betjes and Van IJcken.
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