Globoidnan A, rabdosiin and globoidnan B as new phenolic markers in European-sourced comfrey (Symphytum officinale L.) root samples.
Symphytum officinale L
high-resolution mass spectrometry
phenolic markers
pyrrolizidine alkaloids
rosmarinic acid
Journal
Phytochemical analysis : PCA
ISSN: 1099-1565
Titre abrégé: Phytochem Anal
Pays: England
ID NLM: 9200492
Informations de publication
Date de publication:
Jul 2021
Jul 2021
Historique:
revised:
03
09
2020
received:
20
07
2020
accepted:
07
09
2020
pubmed:
6
10
2020
medline:
4
6
2021
entrez:
5
10
2020
Statut:
ppublish
Résumé
Symphytum officinale L. (comfrey, Boraginaceae) is a cultivated or spontaneously growing medicinal plant that is traditionally used for the treatment of bone fractures, hematomas, muscle pains and joint pains. A wide range of topical preparations and dried roots for ex tempore applications are marketed in European drug stores or pharmacies. The aim of this study was to perform the qualitative and quantitative analysis of pyrrolizidine alkaloids (PAs) and phenolic compounds in the hydroethanolic extracts of 16 commercial comfrey root batches purchased from 12 different European countries. Liquid chromatography hyphenated with high-resolution tandem mass spectrometry (LC-HRMS/MS) was used for the profiling of PAs and phenolic compounds, whereas LC-MS/MS and liquid chromatography with diode array detection (LC-DAD) were used for their quantification. 20 PAs (i.e. intermedine, lycopsamine, acetylintermedine, acetyllycopsamine, symphytine, symphytine-N-oxide), 17 phenolic compounds (i.e. caffeic and rosmarinic acids, rabdosiin, globoidnan A, globoidnan B) and 9 nonphenolic compounds (sugars, organic and fatty acids) were fully or partly annotated in the analysed samples. In addition, the quantitative analyses revealed that globoidnan B, rabdosiin and globoidnan A are new phenolic markers that can be used together with rosmarinic acid and PAs for the quality control of commercial comfrey root batches. This study brings new insights into the phytochemical complexity of S. officinale, revealing not only numerous toxic PAs, but also a significant number of valuable phenolic compounds that could contribute to the bioactivities of comfrey-based preparations.
Substances chimiques
Caffeic Acids
0
Lignans
0
Plant Extracts
0
Pyrrolizidine Alkaloids
0
globoidnan A
0
rabdosiin
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
482-494Informations de copyright
© 2020 The Authors. Phytochemical Analysis published by John Wiley & Sons Ltd.
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