Microbial cell surface display of oxidoreductases: Concepts and applications.
Cell surface display
Oxidoreductases
Whole-cell biocatalysts
Journal
International journal of biological macromolecules
ISSN: 1879-0003
Titre abrégé: Int J Biol Macromol
Pays: Netherlands
ID NLM: 7909578
Informations de publication
Date de publication:
15 Dec 2020
15 Dec 2020
Historique:
received:
25
07
2020
revised:
21
09
2020
accepted:
27
09
2020
pubmed:
6
10
2020
medline:
10
4
2021
entrez:
5
10
2020
Statut:
ppublish
Résumé
Heterologous proteins anchoring on the living cell surface have recently received significant attention due to their promising application potential in various areas of biotechnology. This work presents an overview of displaying strategies for oxidoreductases, enzymes important in applied biocatalysis. Anchoring systems for oxidoreductase display on Gram-positive and Gram-negative bacteria and yeasts were analysed. The effect of cell surface display on enzyme activity and stability was demonstrated. It was also shown that besides the activity and stability improvement, the cell surface display strategy in case of oxidoreductases could solve the problem of cofactor regeneration via co-displaying enzyme cascades. Cell surface displayed oxidoreductase applications were also discussed. It was concluded that the highest potential is in the areas of microbial fuel cells, chemical synthesis, biosensors, and bioremediation.
Identifiants
pubmed: 33017606
pii: S0141-8130(20)34599-2
doi: 10.1016/j.ijbiomac.2020.09.237
pii:
doi:
Substances chimiques
Oxidoreductases
EC 1.-
Types de publication
Journal Article
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
835-841Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.