Molecular characteristics of Staphylococcus aureus associated prosthetic joint infections after hip fractures treated with hemiarthroplasty: a retrospective genome-wide association study.
Aged
Aged, 80 and over
Female
Genome-Wide Association Study
Hemiarthroplasty
/ adverse effects
Hip Fractures
/ mortality
Hip Prosthesis
/ adverse effects
Humans
Male
Norway
Opportunistic Infections
/ complications
Prosthesis-Related Infections
/ complications
Retrospective Studies
Staphylococcal Infections
/ complications
Staphylococcus aureus
/ genetics
Time Factors
Virulence
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
06 10 2020
06 10 2020
Historique:
received:
03
02
2020
accepted:
22
09
2020
entrez:
7
10
2020
pubmed:
8
10
2020
medline:
5
2
2021
Statut:
epublish
Résumé
A retrospective study of Staphylococcus aureus isolates from orthopaedic patients treated between 2000 and 2017 at Akershus University Hospital, Norway was performed using a genome-wide association approach. The aim was to characterize and investigate molecular characteristics unique to S. aureus isolates from HHA associated prosthetic joint infections and potentially explain the HHA patients' elevated 1-year mortality compared to a non-HHA group. The comparison group consisted of patients with non-HHA lower-extremity implant-related S. aureus infections. S. aureus isolates from diagnostic patient samples were whole-genome sequenced. Univariate and multivariate analyses were performed to detect group-associated genetic signatures. A total of 62 HHA patients and 73 non-HHA patients were included. Median age (81 years vs. 74 years; p < 0.001) and 1-year mortality (44% vs. 15%, p < 0.001) were higher in the HHA group. A total of 20 clonal clusters (CCs) were identified; 75% of the isolates consisted of CC45, CC30, CC5, CC15, and CC1. Analyses of core and accessory genome content, including virulence, resistance genes, and k-mer analysis revealed few group-associated variants, none of which could explain the elevated 1-year mortality in HHA patients. Our findings support the premise that all S. aureus can cause invasive infections given the opportunity.
Identifiants
pubmed: 33024212
doi: 10.1038/s41598-020-73736-3
pii: 10.1038/s41598-020-73736-3
pmc: PMC7538562
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
16553Références
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