JMJD3 acts in tandem with KLF4 to facilitate reprogramming to pluripotency.

Animals Catalysis Cell Proliferation Cellular Reprogramming Cellular Senescence Demethylation Enhancer Elements, Genetic / genetics Epithelial Cells / metabolism Fibroblasts / cytology Gene Expression Regulation, Developmental Genome Histones / metabolism Jumonji Domain-Containing Histone Demethylases / metabolism Kruppel-Like Factor 4 Kruppel-Like Transcription Factors / metabolism Lysine / metabolism Mice Models, Biological Pluripotent Stem Cells / cytology Promoter Regions, Genetic Transcriptional Activation / genetics

Journal

Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555

Informations de publication

Date de publication:
08 10 2020
Historique:
received: 22 07 2019
accepted: 16 09 2020
entrez: 9 10 2020
pubmed: 10 10 2020
medline: 21 10 2020
Statut: epublish

Résumé

The interplay between the Yamanaka factors (OCT4, SOX2, KLF4 and c-MYC) and transcriptional/epigenetic co-regulators in somatic cell reprogramming is incompletely understood. Here, we demonstrate that the histone H3 lysine 27 trimethylation (H3K27me3) demethylase JMJD3 plays conflicting roles in mouse reprogramming. On one side, JMJD3 induces the pro-senescence factor Ink4a and degrades the pluripotency regulator PHF20 in a reprogramming factor-independent manner. On the other side, JMJD3 is specifically recruited by KLF4 to reduce H3K27me3 at both enhancers and promoters of epithelial and pluripotency genes. JMJD3 also promotes enhancer-promoter looping through the cohesin loading factor NIPBL and ultimately transcriptional elongation. This competition of forces can be shifted towards improved reprogramming by using early passage fibroblasts or boosting JMJD3's catalytic activity with vitamin C. Our work, thus, establishes a multifaceted role for JMJD3, placing it as a key partner of KLF4 and a scaffold that assists chromatin interactions and activates gene transcription.

Identifiants

DOI: 10.1038/s41467-020-18900-z PMID: 33033262 PMC: PMC7545202
pubmed: 33033262
doi: 10.1038/s41467-020-18900-z
pii: 10.1038/s41467-020-18900-z
pmc: PMC7545202
doi:

Substances chimiques

Histones 0
Klf4 protein, mouse 0
Kruppel-Like Factor 4 0
Kruppel-Like Transcription Factors 0
Jumonji Domain-Containing Histone Demethylases EC 1.14.11.-
Kdm6b protein, mouse EC 1.5.-
Lysine K3Z4F929H6

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

5061

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Auteurs

Yinghua Huang (Y)

Chinese Academy of Sciences (CAS) Key Laboratory of Regenerative Biology and Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health (GIBH), CAS, 510530, Guangzhou, China.
Laboratory of Metabolism and Cell Fate, GIBH, CAS, 510530, Guangzhou, China.
ORCID: 0000-0002-2028-8131

Hui Zhang (H)

Bioland Laboratory (Guangzhou Regenerative Medicine and Health Guangdong Laboratory), 510005, Guangzhou, China.

Lulu Wang (L)

Chinese Academy of Sciences (CAS) Key Laboratory of Regenerative Biology and Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health (GIBH), CAS, 510530, Guangzhou, China.
Laboratory of Metabolism and Cell Fate, GIBH, CAS, 510530, Guangzhou, China.
ORCID: 0000-0002-8711-5876

Chuanqing Tang (C)

Chinese Academy of Sciences (CAS) Key Laboratory of Regenerative Biology and Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health (GIBH), CAS, 510530, Guangzhou, China.
Laboratory of Metabolism and Cell Fate, GIBH, CAS, 510530, Guangzhou, China.
ORCID: 0000-0001-5513-2340

Xiaogan Qin (X)

Chinese Academy of Sciences (CAS) Key Laboratory of Regenerative Biology and Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health (GIBH), CAS, 510530, Guangzhou, China.
Laboratory of Metabolism and Cell Fate, GIBH, CAS, 510530, Guangzhou, China.

Xinyu Wu (X)

Chinese Academy of Sciences (CAS) Key Laboratory of Regenerative Biology and Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health (GIBH), CAS, 510530, Guangzhou, China.
Laboratory of Metabolism and Cell Fate, GIBH, CAS, 510530, Guangzhou, China.
University of Chinese Academy of Sciences, 100049, Beijing, China.
ORCID: 0000-0002-5293-5479

Meifang Pan (M)

Chinese Academy of Sciences (CAS) Key Laboratory of Regenerative Biology and Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health (GIBH), CAS, 510530, Guangzhou, China.
Laboratory of Metabolism and Cell Fate, GIBH, CAS, 510530, Guangzhou, China.
University of Chinese Academy of Sciences, 100049, Beijing, China.

Yujia Tang (Y)

Chinese Academy of Sciences (CAS) Key Laboratory of Regenerative Biology and Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health (GIBH), CAS, 510530, Guangzhou, China.
Laboratory of Metabolism and Cell Fate, GIBH, CAS, 510530, Guangzhou, China.

Zhongzhou Yang (Z)

Chinese Academy of Sciences (CAS) Key Laboratory of Regenerative Biology and Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health (GIBH), CAS, 510530, Guangzhou, China.

Isaac A Babarinde (IA)

Department of Biology, Southern University of Science and Technology, 518055, Shenzhen, China.

Runxia Lin (R)

Chinese Academy of Sciences (CAS) Key Laboratory of Regenerative Biology and Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health (GIBH), CAS, 510530, Guangzhou, China.
Laboratory of Metabolism and Cell Fate, GIBH, CAS, 510530, Guangzhou, China.
University of Chinese Academy of Sciences, 100049, Beijing, China.

Guanyu Ji (G)

Science and Technology Department, E-GENE, 518118, Shenzhen, China.
ORCID: 0000-0001-9818-8694

Yiwei Lai (Y)

Chinese Academy of Sciences (CAS) Key Laboratory of Regenerative Biology and Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health (GIBH), CAS, 510530, Guangzhou, China.
Laboratory of Integrative Biology, GIBH, CAS, 510530, Guangzhou, China.
ORCID: 0000-0003-0733-812X

Xueting Xu (X)

Chinese Academy of Sciences (CAS) Key Laboratory of Regenerative Biology and Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health (GIBH), CAS, 510530, Guangzhou, China.
Laboratory of Metabolism and Cell Fate, GIBH, CAS, 510530, Guangzhou, China.
School of Life Sciences, University of Science and Technology of China, 230027, Hefei, China.
ORCID: 0000-0002-8011-7449

Jianbin Su (J)

Chinese Academy of Sciences (CAS) Key Laboratory of Regenerative Biology and Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health (GIBH), CAS, 510530, Guangzhou, China.
Laboratory of Metabolism and Cell Fate, GIBH, CAS, 510530, Guangzhou, China.

Xue Wen (X)

Stem Cell and Cancer Center, First Affiliated Hospital, Jilin University, 130061, Changchun, China.

Takashi Satoh (T)

Department of Host Defense, Research Institute for Microbial Diseases, Osaka University, Osaka, 565-0871, Japan.

Tanveer Ahmed (T)

Chinese Academy of Sciences (CAS) Key Laboratory of Regenerative Biology and Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health (GIBH), CAS, 510530, Guangzhou, China.
Laboratory of Metabolism and Cell Fate, GIBH, CAS, 510530, Guangzhou, China.
ORCID: 0000-0003-0519-2315

Vikas Malik (V)

Chinese Academy of Sciences (CAS) Key Laboratory of Regenerative Biology and Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health (GIBH), CAS, 510530, Guangzhou, China.
Laboratory of Integrative Biology, GIBH, CAS, 510530, Guangzhou, China.
ORCID: 0000-0002-9300-6414

Carl Ward (C)

Chinese Academy of Sciences (CAS) Key Laboratory of Regenerative Biology and Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health (GIBH), CAS, 510530, Guangzhou, China.
Laboratory of Integrative Biology, GIBH, CAS, 510530, Guangzhou, China.
ORCID: 0000-0003-0889-9025

Giacomo Volpe (G)

Chinese Academy of Sciences (CAS) Key Laboratory of Regenerative Biology and Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health (GIBH), CAS, 510530, Guangzhou, China.
Laboratory of Integrative Biology, GIBH, CAS, 510530, Guangzhou, China.
ORCID: 0000-0001-5000-6951

Lin Guo (L)

Chinese Academy of Sciences (CAS) Key Laboratory of Regenerative Biology and Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health (GIBH), CAS, 510530, Guangzhou, China.
ORCID: 0000-0001-5698-270X

Jinlong Chen (J)

Chinese Academy of Sciences (CAS) Key Laboratory of Regenerative Biology and Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health (GIBH), CAS, 510530, Guangzhou, China.

Li Sun (L)

Department of Biology, Southern University of Science and Technology, 518055, Shenzhen, China.

Yingying Li (Y)

Bioland Laboratory (Guangzhou Regenerative Medicine and Health Guangdong Laboratory), 510005, Guangzhou, China.

Xiaofen Huang (X)

Chinese Academy of Sciences (CAS) Key Laboratory of Regenerative Biology and Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health (GIBH), CAS, 510530, Guangzhou, China.

Xichen Bao (X)

Chinese Academy of Sciences (CAS) Key Laboratory of Regenerative Biology and Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health (GIBH), CAS, 510530, Guangzhou, China.
Bioland Laboratory (Guangzhou Regenerative Medicine and Health Guangdong Laboratory), 510005, Guangzhou, China.
Joint School of Life Sciences, GIBH and Guangzhou Medical University, 511436, Guangzhou, China.
ORCID: 0000-0003-0389-4233

Fei Gao (F)

Science and Technology Department, E-GENE, 518118, Shenzhen, China.
Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, 518120, Shenzhen, China.

Baohua Liu (B)

Health Science Center, Shenzhen University, 518060, Shenzhen, China.
ORCID: 0000-0002-1599-8059

Hui Zheng (H)

Chinese Academy of Sciences (CAS) Key Laboratory of Regenerative Biology and Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health (GIBH), CAS, 510530, Guangzhou, China.
Bioland Laboratory (Guangzhou Regenerative Medicine and Health Guangdong Laboratory), 510005, Guangzhou, China.
Joint School of Life Sciences, GIBH and Guangzhou Medical University, 511436, Guangzhou, China.
ORCID: 0000-0001-6801-0529

Ralf Jauch (R)

School of Biomedical Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, SAR, China.
ORCID: 0000-0002-6590-9579

Liangxue Lai (L)

Chinese Academy of Sciences (CAS) Key Laboratory of Regenerative Biology and Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health (GIBH), CAS, 510530, Guangzhou, China.
Bioland Laboratory (Guangzhou Regenerative Medicine and Health Guangdong Laboratory), 510005, Guangzhou, China.
Joint School of Life Sciences, GIBH and Guangzhou Medical University, 511436, Guangzhou, China.

Guangjin Pan (G)

Chinese Academy of Sciences (CAS) Key Laboratory of Regenerative Biology and Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health (GIBH), CAS, 510530, Guangzhou, China.
Bioland Laboratory (Guangzhou Regenerative Medicine and Health Guangdong Laboratory), 510005, Guangzhou, China.
Joint School of Life Sciences, GIBH and Guangzhou Medical University, 511436, Guangzhou, China.
ORCID: 0000-0002-2378-7198

Jiekai Chen (J)

Chinese Academy of Sciences (CAS) Key Laboratory of Regenerative Biology and Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health (GIBH), CAS, 510530, Guangzhou, China.
Bioland Laboratory (Guangzhou Regenerative Medicine and Health Guangdong Laboratory), 510005, Guangzhou, China.
Joint School of Life Sciences, GIBH and Guangzhou Medical University, 511436, Guangzhou, China.
ORCID: 0000-0001-5168-7074

Giuseppe Testa (G)

Department of Experimental Oncology, European Institute of Oncology, Milan, 20139, Italy.
ORCID: 0000-0002-9104-0918

Shizuo Akira (S)

Department of Host Defense, Research Institute for Microbial Diseases, Osaka University, Osaka, 565-0871, Japan.

Jifan Hu (J)

Stem Cell and Cancer Center, First Affiliated Hospital, Jilin University, 130061, Changchun, China.
ORCID: 0000-0002-2174-0361

Duanqing Pei (D)

Chinese Academy of Sciences (CAS) Key Laboratory of Regenerative Biology and Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health (GIBH), CAS, 510530, Guangzhou, China.
Bioland Laboratory (Guangzhou Regenerative Medicine and Health Guangdong Laboratory), 510005, Guangzhou, China.
ORCID: 0000-0002-5222-014X

Andrew P Hutchins (AP)

Department of Biology, Southern University of Science and Technology, 518055, Shenzhen, China.
ORCID: 0000-0001-7784-2255

Miguel A Esteban (MA)

Chinese Academy of Sciences (CAS) Key Laboratory of Regenerative Biology and Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health (GIBH), CAS, 510530, Guangzhou, China. miguel@gibh.ac.cn.
Laboratory of Integrative Biology, GIBH, CAS, 510530, Guangzhou, China. miguel@gibh.ac.cn.
Joint School of Life Sciences, GIBH and Guangzhou Medical University, 511436, Guangzhou, China. miguel@gibh.ac.cn.
Institute for Stem Cells and Regeneration, CAS, 100101, Beijing, China. miguel@gibh.ac.cn.
ORCID: 0000-0002-1426-6809

Baoming Qin (B)

Chinese Academy of Sciences (CAS) Key Laboratory of Regenerative Biology and Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health (GIBH), CAS, 510530, Guangzhou, China. qin_baoming@gibh.ac.cn.
Laboratory of Metabolism and Cell Fate, GIBH, CAS, 510530, Guangzhou, China. qin_baoming@gibh.ac.cn.
Bioland Laboratory (Guangzhou Regenerative Medicine and Health Guangdong Laboratory), 510005, Guangzhou, China. qin_baoming@gibh.ac.cn.
Joint School of Life Sciences, GIBH and Guangzhou Medical University, 511436, Guangzhou, China. qin_baoming@gibh.ac.cn.
ORCID: 0000-0001-7652-161X

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › JMJD3 acts in tandem with KLF4 to facilitate...
questionsmedicales.fr › Phénomènes chimiques › Catalyse › JMJD3 acts in tandem with KLF4 to facilitate...
questionsmedicales.fr › Phénomènes physiologiques › Croissance et développement › Croissance › Processus de croissance cellulaire › Prolifération cellulaire › JMJD3 acts in tandem with KLF4 to facilitate...
questionsmedicales.fr › Phénomènes génétiques › Reprogrammation cellulaire › JMJD3 acts in tandem with KLF4 to facilitate...
questionsmedicales.fr › Phénomènes physiologiques › Croissance et développement › Vieillissement › Vieillissement de la cellule › JMJD3 acts in tandem with KLF4 to facilitate...
questionsmedicales.fr › Métabolisme › Désalkylation › Déméthylation › JMJD3 acts in tandem with KLF4 to facilitate...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Composants de génome › Gènes › Composants de gène › Éléments de régulation transcriptionnelle › Éléments activateurs (génétique) › JMJD3 acts in tandem with KLF4 to facilitate...
questionsmedicales.fr › Cellules › Cellules épithéliales › JMJD3 acts in tandem with KLF4 to facilitate...
questionsmedicales.fr › Cellules › Cellules du tissu conjonctif › Fibroblastes › JMJD3 acts in tandem with KLF4 to facilitate...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Régulation de l'expression des gènes au cours du développement › JMJD3 acts in tandem with KLF4 to facilitate...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › JMJD3 acts in tandem with KLF4 to facilitate...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Nucléoprotéines › Histone › JMJD3 acts in tandem with KLF4 to facilitate...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Oxidoreductases › Oxygénases › Dioxygenases › Jumonji Domain-Containing Histone Demethylases › JMJD3 acts in tandem with KLF4 to facilitate...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Facteurs de transcription › Facteurs de transcription Krüppel-like › Facteur-4 de type Kruppel › JMJD3 acts in tandem with KLF4 to facilitate...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Facteurs de transcription › Facteurs de transcription Krüppel-like › JMJD3 acts in tandem with KLF4 to facilitate...
questionsmedicales.fr › Acides aminés, peptides et protéines › Acides aminés › Acides aminés essentiels › Lysine › JMJD3 acts in tandem with KLF4 to facilitate...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › JMJD3 acts in tandem with KLF4 to facilitate...
questionsmedicales.fr › Techniques d'investigation › Modèles théoriques › Modèles biologiques › JMJD3 acts in tandem with KLF4 to facilitate...
questionsmedicales.fr › Cellules › Cellules souches › Cellules souches pluripotentes › JMJD3 acts in tandem with KLF4 to facilitate...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Composants de génome › Gènes › Composants de gène › Éléments de régulation transcriptionnelle › Régions promotrices (génétique) › JMJD3 acts in tandem with KLF4 to facilitate...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Activation de la transcription › JMJD3 acts in tandem with KLF4 to facilitate...