Whole genome sequencing and phylogenetic classification of Tunisian SARS-CoV-2 strains from patients of the Military Hospital in Tunis.
Adult
Asymptomatic Diseases
COVID-19
/ diagnosis
Europe
/ epidemiology
Female
Genome, Viral
Hospitals, Military
Humans
Male
Middle Aged
Military Personnel
Morocco
/ epidemiology
Pandemics
Pedigree
Phylogeny
RNA, Viral
/ genetics
SARS-CoV-2
/ classification
Travel-Related Illness
Tunisia
/ epidemiology
Viral Load
Whole Genome Sequencing
COVID-19
Morocco
RT-qPCR
SARS-CoV-2
Sequencing
Tunisia
Journal
Virus genes
ISSN: 1572-994X
Titre abrégé: Virus Genes
Pays: United States
ID NLM: 8803967
Informations de publication
Date de publication:
Dec 2020
Dec 2020
Historique:
received:
02
07
2020
accepted:
12
09
2020
pubmed:
10
10
2020
medline:
15
12
2020
entrez:
9
10
2020
Statut:
ppublish
Résumé
In the present work, two complete genome sequences of SARS-CoV-2 were obtained from nasal swab samples of Tunisian SARS-CoV-2 PCR-positive patients using nanopore sequencing. The virus genomes of two of the patients examined, a Tunisian soldier returning from a mission in Morocco and a member of another Tunisian family, showed significant differences in analyses of the total genome and single nucleotide polymorphisms (SNPs). Phylogenetic relationships with known SARS-CoV-2 genomes in the African region, some European and Middle Eastern countries and initial epidemiological conclusions indicate that the introduction of SARS-CoV-2 into Tunisia from two independent sources was travel-related.
Identifiants
pubmed: 33034798
doi: 10.1007/s11262-020-01795-9
pii: 10.1007/s11262-020-01795-9
pmc: PMC7545018
doi:
Substances chimiques
RNA, Viral
0
Types de publication
Case Reports
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
767-771Subventions
Organisme : Ertüchtigungsinitiative der Deutschen Bundesregierung
ID : OR12-370.43 ERT TUN IMB
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