Fast diagnostic test for familial Mediterranean fever based on a kinase inhibitor.
Adolescent
Adult
Aged
Aged, 80 and over
Arthritis, Juvenile
/ diagnosis
Behcet Syndrome
/ diagnosis
Case-Control Studies
Child
Child, Preschool
Cryopyrin-Associated Periodic Syndromes
/ diagnosis
Familial Mediterranean Fever
/ diagnosis
Female
Fever
/ diagnosis
Fever of Unknown Origin
/ diagnosis
Hereditary Autoinflammatory Diseases
/ diagnosis
Humans
Immunologic Tests
/ methods
Inflammasomes
/ drug effects
Interleukin-1beta
/ drug effects
Lupus Erythematosus, Systemic
/ diagnosis
Male
Mevalonate Kinase Deficiency
/ diagnosis
Middle Aged
Monocytes
/ drug effects
Protein Kinase Inhibitors
/ pharmacology
Pyrin
/ drug effects
Pyroptosis
/ drug effects
Sensitivity and Specificity
Sepsis
/ diagnosis
Staurosporine
/ analogs & derivatives
Still's Disease, Adult-Onset
/ diagnosis
Young Adult
cytokines
familial mediterranean fever
inflammation
Journal
Annals of the rheumatic diseases
ISSN: 1468-2060
Titre abrégé: Ann Rheum Dis
Pays: England
ID NLM: 0372355
Informations de publication
Date de publication:
01 2021
01 2021
Historique:
received:
19
06
2020
revised:
27
08
2020
accepted:
30
08
2020
pubmed:
11
10
2020
medline:
9
2
2021
entrez:
10
10
2020
Statut:
ppublish
Résumé
Familial Mediterranean fever (FMF) is the most frequent hereditary autoinflammatory disease. Its diagnosis relies on a set of clinical criteria and a genetic confirmation on identification of biallelic pathogenic Real-time pyroptosis and IL-1β secretion were monitored in response to UCN-01 in monocytes from FMF patients (n=67), HD (n=71) and OID patients (n=40). Sensitivity and specificity of the resulting diagnostic tests were determined by receiver operating characteristic curve analyses. Inflammasome monitoring in response to UCN-01 discriminates FMF patients from other individuals. Pyroptosis assessment leads to a fast FMF diagnosis while combining pyroptosis and IL-1β dosage renders UCN-01-based assays highly sensitive and specific. UCN-01-triggered monocytes responses were influenced by UCN-01-based inflammasome assays could be used to rapidly diagnose FMF, with high sensitivity and specificity.
Sections du résumé
BACKGROUND AND OBJECTIVE
Familial Mediterranean fever (FMF) is the most frequent hereditary autoinflammatory disease. Its diagnosis relies on a set of clinical criteria and a genetic confirmation on identification of biallelic pathogenic
METHODS
Real-time pyroptosis and IL-1β secretion were monitored in response to UCN-01 in monocytes from FMF patients (n=67), HD (n=71) and OID patients (n=40). Sensitivity and specificity of the resulting diagnostic tests were determined by receiver operating characteristic curve analyses.
RESULTS
Inflammasome monitoring in response to UCN-01 discriminates FMF patients from other individuals. Pyroptosis assessment leads to a fast FMF diagnosis while combining pyroptosis and IL-1β dosage renders UCN-01-based assays highly sensitive and specific. UCN-01-triggered monocytes responses were influenced by
CONCLUSIONS
UCN-01-based inflammasome assays could be used to rapidly diagnose FMF, with high sensitivity and specificity.
Identifiants
pubmed: 33037005
pii: annrheumdis-2020-218366
doi: 10.1136/annrheumdis-2020-218366
doi:
Substances chimiques
IL1B protein, human
0
Inflammasomes
0
Interleukin-1beta
0
MEFV protein, human
0
Protein Kinase Inhibitors
0
Pyrin
0
7-hydroxystaurosporine
7BU5H4V94A
Staurosporine
H88EPA0A3N
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
128-132Informations de copyright
© Author(s) (or their employer(s)) 2021. No commercial re-use. See rights and permissions. Published by BMJ.
Déclaration de conflit d'intérêts
Competing interests: YJ, FM, AB, AM and TH are coinventors and owners of a patent 'Methods and kits for diagnosis of familial Mediterranean fever' (WO/2019/048569).