Sugar transport for enhanced xylose utilization in Ashbya gossypii.
Ashbya gossypii
CRISPR/Cas9
Glucose
Sugar transport
Xylose
Journal
Journal of industrial microbiology & biotechnology
ISSN: 1476-5535
Titre abrégé: J Ind Microbiol Biotechnol
Pays: Germany
ID NLM: 9705544
Informations de publication
Date de publication:
Dec 2020
Dec 2020
Historique:
received:
17
07
2020
accepted:
03
10
2020
pubmed:
11
10
2020
medline:
2
4
2021
entrez:
10
10
2020
Statut:
ppublish
Résumé
The co-utilization of mixed (pentose/hexose) sugars constitutes a challenge for microbial fermentations. The fungus Ashbya gossypii, which is currently exploited for the industrial production of riboflavin, has been presented as an efficient biocatalyst for the production of biolipids using xylose-rich substrates. However, the utilization of xylose in A. gossypii is hindered by hexose sugars. Three A. gossypii homologs (AFL204C, AFL205C and AFL207C) of the yeast HXT genes that code for hexose transporters have been identified and characterized by gene-targeting approaches. Significant differences in the expression profile of the HXT homologs were found in response to different concentrations of sugars. More importantly, an amino acid replacement (N355V) in AFL205Cp, introduced by CRISPR/Cas9-mediated genomic edition, notably enhanced the utilization of xylose in the presence of glucose. Hence, the introduction of the afl205c-N355V allele in engineered strains of A. gossypii will further benefit the utilization of mixed sugars in this fungus.
Identifiants
pubmed: 33037458
doi: 10.1007/s10295-020-02320-5
pii: 10.1007/s10295-020-02320-5
pmc: PMC7728639
doi:
Substances chimiques
Monosaccharide Transport Proteins
0
Pentoses
0
Xylose
A1TA934AKO
Glucose
IY9XDZ35W2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1173-1179Subventions
Organisme : Agencia Estatal de Investigación
ID : BIO2017-88435-R
Organisme : Consejería de Educación, Junta de Castilla y León
ID : SA016P17
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