Interplay of MPP5a with Rab11 synergistically builds epithelial apical polarity and zonula adherens.

Adherens Junctions / genetics Animals Cadherins / genetics Cell Movement / physiology Cell Polarity / physiology Epithelial Cells Golgi Apparatus / genetics Guanylate Cyclase / genetics Protein Transport / physiology Zebrafish / embryology Zebrafish Proteins / genetics rab GTP-Binding Proteins / genetics
Adherens junctions remodeling Apical polarity MPP5a Rab11 Zonula adherens

Journal

Development (Cambridge, England)
ISSN: 1477-9129
Titre abrégé: Development
Pays: England
ID NLM: 8701744

Informations de publication

Date de publication:
19 11 2020
Historique:
received: 02 09 2019
accepted: 09 10 2020
pubmed: 17 10 2020
medline: 20 1 2021
entrez: 16 10 2020
Statut: epublish

Résumé

Adherens junction remodeling regulated by apical polarity proteins constitutes a major driving force for tissue morphogenesis, although the precise mechanism remains inconclusive. Here, we report that, in zebrafish, the Crumbs complex component MPP5a interacts with small GTPase Rab11 in Golgi to transport cadherin and Crumbs components synergistically to the apical domain, thus establishing apical epithelial polarity and adherens junctions. In contrast, Par complex recruited by MPP5a is incapable of interacting with Rab11 but might assemble cytoskeleton to facilitate cadherin exocytosis. In accordance, dysfunction of MPP5a induces an invasive migration of epithelial cells. This adherens junction remodeling pattern is frequently observed in zebrafish lens epithelial cells and neuroepithelial cells. The data identify an unrecognized MPP5a-Rab11 complex and describe its essential role in guiding apical polarization and zonula adherens formation in epithelial cells.

Identifiants

DOI: 10.1242/dev.184457 PMID: 33060129
pubmed: 33060129
pii: dev.184457
doi: 10.1242/dev.184457
pii:
doi:

Substances chimiques

Cadherins 0
Zebrafish Proteins 0
mpp5a protein, zebrafish EC 2.7.4.8
rab11 protein EC 3.6.1.-
rab GTP-Binding Proteins EC 3.6.5.2
Guanylate Cyclase EC 4.6.1.2

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Informations de copyright

© 2020. Published by The Company of Biologists Ltd.

Déclaration de conflit d'intérêts

Competing interestsThe authors declare no competing or financial interests.

Auteurs

Yumei Hao (Y)

Eye Center of the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310058, China.
The Institute of Translational Medicine, Zhejiang University, Hangzhou 310058, China.

Yao Zhou (Y)

Eye Center of the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310058, China.
The Institute of Translational Medicine, Zhejiang University, Hangzhou 310058, China.

Yinhui Yu (Y)

Eye Center of the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310058, China.

Mingjie Zheng (M)

Eye Center of the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310058, China.
The Institute of Translational Medicine, Zhejiang University, Hangzhou 310058, China.

Kechao Weng (K)

Eye Center of the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310058, China.
The Institute of Translational Medicine, Zhejiang University, Hangzhou 310058, China.

Ziqi Kou (Z)

Eye Center of the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310058, China.
The Institute of Translational Medicine, Zhejiang University, Hangzhou 310058, China.
ORCID: 0000-0003-3857-4299

Jiancheng Liang (J)

Eye Center of the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310058, China.
The Institute of Translational Medicine, Zhejiang University, Hangzhou 310058, China.

Qian Zhang (Q)

Life Sciences Institute and Innovation Center for Cell Signaling Network, Zhejiang University, Hangzhou 310058, China.

Xiajing Tang (X)

Eye Center of the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310058, China.

Pinglong Xu (P)

Life Sciences Institute and Innovation Center for Cell Signaling Network, Zhejiang University, Hangzhou 310058, China.
ORCID: 0000-0001-7726-5443

Brian A Link (BA)

Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin, Milwaukee, WI 53226, USA.

Ke Yao (K)

Eye Center of the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310058, China jianzou@zju.edu.cn xlren@zju.edu.cn.
Zhejiang Provincial Key Lab of Ophthalmology, Hangzhou 310058, China.

Jian Zou (J)

Eye Center of the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310058, China jianzou@zju.edu.cn xlren@zju.edu.cn.
The Institute of Translational Medicine, Zhejiang University, Hangzhou 310058, China.
Zhejiang Provincial Key Lab of Ophthalmology, Hangzhou 310058, China.
ORCID: 0000-0002-8884-9978

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Classifications MeSH

questionsmedicales.fr › Cellules › Structures cellulaires › Membrane cellulaire › Structures de la membrane cellulaire › Jonctions intercellulaires › Jonctions adhérentes › Interplay of MPP5a with Rab11 synergistically...
questionsmedicales.fr › Eucaryotes › Animaux › Interplay of MPP5a with Rab11 synergistically...
questionsmedicales.fr › Facteurs biologiques › Antigènes › Antigènes de surface › Molécules d'adhérence cellulaire › Cadhérines › Interplay of MPP5a with Rab11 synergistically...
questionsmedicales.fr › Phénomènes physiologiques › Mouvement cellulaire › Interplay of MPP5a with Rab11 synergistically...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Polarité de la cellule › Interplay of MPP5a with Rab11 synergistically...
questionsmedicales.fr › Cellules › Cellules épithéliales › Interplay of MPP5a with Rab11 synergistically...
questionsmedicales.fr › Cellules › Structures cellulaires › Espace intracellulaire › Cytoplasme › Structures cytoplasmiques › Organites › Appareil de Golgi › Interplay of MPP5a with Rab11 synergistically...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines et peptides de signalisation intracellulaire › Guanylate cyclase › Interplay of MPP5a with Rab11 synergistically...
questionsmedicales.fr › Métabolisme › Transport biologique › Transport des protéines › Interplay of MPP5a with Rab11 synergistically...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Poissons › Cypriniformes › Cyprinidae › Danio zébré › Interplay of MPP5a with Rab11 synergistically...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines de poisson › Protéines de poisson-zèbre › Interplay of MPP5a with Rab11 synergistically...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines et peptides de signalisation intracellulaire › Protéines G › Protéines G monomériques › Protéines G rab › Interplay of MPP5a with Rab11 synergistically...