Biological characteristics and genome analysis of a novel phage vB_KpnP_IME279 infecting Klebsiella pneumoniae.
Anti-Bacterial Agents
/ pharmacology
Bacterial Typing Techniques
Bacteriophages
/ classification
Base Composition
Drug Resistance, Multiple, Bacterial
Genome, Viral
Hospitals
Host Specificity
Humans
Klebsiella pneumoniae
/ drug effects
Microbiological Techniques
Multilocus Sequence Typing
Phylogeny
Podoviridae
/ classification
RNA, Ribosomal, 16S
Sewage
/ microbiology
Temperature
Whole Genome Sequencing
Journal
Folia microbiologica
ISSN: 1874-9356
Titre abrégé: Folia Microbiol (Praha)
Pays: United States
ID NLM: 0376757
Informations de publication
Date de publication:
Dec 2020
Dec 2020
Historique:
received:
28
08
2019
accepted:
19
01
2020
pubmed:
17
10
2020
medline:
7
7
2021
entrez:
16
10
2020
Statut:
ppublish
Résumé
Klebsiella pneumoniae (family Enterobacteriaceae) is a gram-negative bacterium that has strong pathogenicity to humans and can cause sepsis, pneumonia, and urinary tract infection. In recent years, the unreasonable use of antibacterial drugs has led to an increase in drug-resistant strains of K. pneumoniae, a serious threat to public health. Bacteriophages, viruses that infect bacteria, are ubiquitous in the natural environment. They are considered to be the most promising substitute for antibiotics because of their high specificity, high efficiency, high safety, low cost, and short development cycle. In this study, a novel phage designated vB_KpnP_IME279 was successfully isolated from hospital sewage using a multidrug-resistant strain of K. pneumoniae as an indicator. A one-step growth curve showed that vB_KpnP_IME279 has a burst size of 140 plaque-forming units/cell and a latent period of 20 min at its optimal multiplicity of infection (MOI = 0.1). Phage vB_KpnP_IME279 survives in a wide pH range between 3 and 11 and is stable at temperatures ranging from 40 to 60 °C. Ten of the 20 strains of K. pneumoniae including the host bacteria were lysed by the phage vB_KpnP_IME279, and the multilocus sequence typing and wzi typing of the 10 strains were ST11, ST37, ST375, wzi209, wzi52, and wzi72, respectively. The genome of vB_KpnP_IME279 is 42,518 bp long with a G + C content of 59.3%. Electron microscopic observation showed that the phage belongs to the family Podoviridae. BLASTN alignment showed that the genome of the phage has low similarity with currently known phages. The evolutionary relationship between phage vB_KpnP_IME279 and other Podoviridae was analyzed using a phylogenetic tree based on sequences of phage major capsid protein and indicates that the phage vB_KpnP_IME279 belongs to the Podoviridae subfamily. These data enhance understanding of K. pneumoniae phages and will help in development of treatments for multidrug-resistant bacteria using phages.
Identifiants
pubmed: 33064268
doi: 10.1007/s12223-020-00775-8
pii: 10.1007/s12223-020-00775-8
doi:
Substances chimiques
Anti-Bacterial Agents
0
RNA, Ribosomal, 16S
0
Sewage
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
925-936Subventions
Organisme : The National Key Research and Development Program of China
ID : 2016YFC1202705,AWS16J020 and AWS15J006
Organisme : the National Natural Science Foundation of China
ID : 81572045, 81672001, and 81621005
Organisme : the Fundamental Research Funds for the Central Universities and Research Projects on Biomedical Transformation of China-Japan Friendship Hospital
ID : PYBZ1820
Organisme : National Science and Technology Major Project
ID : 2018ZX10201001
Organisme : National Natural Science Foundation of China.
ID : 31900489
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