miRNA repertoires of cystic fibrosis ex vivo models highlight miR-181a and miR-101 that regulate WISP1 expression.
WISP1
cystic fibrosis
gene therapy
isomiR
lung disease
miR-101
miR-181a
miRNA
small RNAseq
wound healing
Journal
The Journal of pathology
ISSN: 1096-9896
Titre abrégé: J Pathol
Pays: England
ID NLM: 0204634
Informations de publication
Date de publication:
02 2021
02 2021
Historique:
received:
25
06
2019
revised:
24
09
2020
accepted:
19
10
2020
pubmed:
24
10
2020
medline:
8
6
2021
entrez:
23
10
2020
Statut:
ppublish
Résumé
Cystic fibrosis (CF), a genetic disorder, is characterized by chronic lung disease. Small non-coding RNAs are key regulators of gene expression and participate in various processes, which are dysregulated in CF; however, they remain poorly studied. Here, we determined the complete microRNAs (miRNAs) expression pattern in three CF ex vivo models. The miRNA profiles of air-liquid interface cultures of airway epithelia (bronchi, nasal cells, and nasal polyps) samples from patients with CF and non-CF controls were obtained by deep sequencing. Compared with non-CF controls, several miRNAs were deregulated in CF samples; for instance, miR-181a-5p and the miR-449 family were upregulated. Moreover, mature miRNAs often showed variations (i.e. isomiRs) relative to their reference sequence, such as miR-101, suggesting that miRNAs consist of heterogeneous repertoires of multiple isoforms with different effects on gene expression. Analysis of miR-181a-5p and miR-101-3p roles indicated that they regulate the expression of WISP1, a key component of cell proliferation/migration programs. We showed that miR-101 and miR-181a-5p participated in aberrant recapitulation of wound healing programs by controlling WISP1 mRNA and protein level. Our miRNA expression data bring new insights into CF physiopathology and define new potential therapeutic targets in CF. © 2020 The Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
Substances chimiques
CCN Intercellular Signaling Proteins
0
CCN4 protein, human
0
MIRN101 microRNA, human
0
MIrn181 microRNA, human
0
MicroRNAs
0
Proto-Oncogene Proteins
0
RNA, Messenger
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
186-197Subventions
Organisme : CIHR
ID : PJT148593
Pays : Canada
Informations de copyright
© 2020 The Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
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