Genetic analysis reveals unique characteristics of Plasmodium falciparum parasite populations in Haiti.
Genetic analysis
Haiti
Hispaniola
Malaria elimination
Plasmodium falciparum
Population genetics
Journal
Malaria journal
ISSN: 1475-2875
Titre abrégé: Malar J
Pays: England
ID NLM: 101139802
Informations de publication
Date de publication:
23 Oct 2020
23 Oct 2020
Historique:
received:
25
06
2020
accepted:
03
10
2020
entrez:
24
10
2020
pubmed:
25
10
2020
medline:
1
6
2021
Statut:
epublish
Résumé
With increasing interest in eliminating malaria from the Caribbean region, Haiti is one of the two countries on the island of Hispaniola with continued malaria transmission. While the Haitian population remains at risk for malaria, there are a limited number of cases annually, making conventional epidemiological measures such as case incidence and prevalence of potentially limited value for fine-scale resolution of transmission patterns and trends. In this context, genetic signatures may be useful for the identification and characterization of the Plasmodium falciparum parasite population in order to identify foci of transmission, detect outbreaks, and track parasite movement to potentially inform malaria control and elimination strategies. This study evaluated the genetic signals based on analysis of 21 single-nucleotide polymorphisms (SNPs) from 462 monogenomic (single-genome) P. falciparum DNA samples extracted from dried blood spots collected from malaria-positive patients reporting to health facilities in three southwestern Haitian departments (Nippes, Grand'Anse, and Sud) in 2016. Assessment of the parasite genetic relatedness revealed evidence of clonal expansion within Nippes and the exchange of parasite lineages between Nippes, Sud, and Grand'Anse. Furthermore, 437 of the 462 samples shared high levels of genetic similarity-at least 20 of 21 SNPS-with at least one other sample in the dataset. These results revealed patterns of relatedness suggestive of the repeated recombination of a limited number of founding parasite types without significant outcrossing. These genetic signals offer clues to the underlying relatedness of parasite populations and may be useful for the identification of the foci of transmission and tracking of parasite movement in Haiti for malaria elimination.
Sections du résumé
BACKGROUND
BACKGROUND
With increasing interest in eliminating malaria from the Caribbean region, Haiti is one of the two countries on the island of Hispaniola with continued malaria transmission. While the Haitian population remains at risk for malaria, there are a limited number of cases annually, making conventional epidemiological measures such as case incidence and prevalence of potentially limited value for fine-scale resolution of transmission patterns and trends. In this context, genetic signatures may be useful for the identification and characterization of the Plasmodium falciparum parasite population in order to identify foci of transmission, detect outbreaks, and track parasite movement to potentially inform malaria control and elimination strategies.
METHODS
METHODS
This study evaluated the genetic signals based on analysis of 21 single-nucleotide polymorphisms (SNPs) from 462 monogenomic (single-genome) P. falciparum DNA samples extracted from dried blood spots collected from malaria-positive patients reporting to health facilities in three southwestern Haitian departments (Nippes, Grand'Anse, and Sud) in 2016.
RESULTS
RESULTS
Assessment of the parasite genetic relatedness revealed evidence of clonal expansion within Nippes and the exchange of parasite lineages between Nippes, Sud, and Grand'Anse. Furthermore, 437 of the 462 samples shared high levels of genetic similarity-at least 20 of 21 SNPS-with at least one other sample in the dataset.
CONCLUSIONS
CONCLUSIONS
These results revealed patterns of relatedness suggestive of the repeated recombination of a limited number of founding parasite types without significant outcrossing. These genetic signals offer clues to the underlying relatedness of parasite populations and may be useful for the identification of the foci of transmission and tracking of parasite movement in Haiti for malaria elimination.
Identifiants
pubmed: 33097045
doi: 10.1186/s12936-020-03439-7
pii: 10.1186/s12936-020-03439-7
pmc: PMC7583211
doi:
Substances chimiques
DNA, Protozoan
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
379Subventions
Organisme : Bill & Melinda Gates Foundation
ID : INV-008799
Pays : United States
Références
Malar J. 2015 Jun 05;14:237
pubmed: 26043728
Genome Med. 2017 Jan 24;9(1):5
pubmed: 28118860
PLoS One. 2011;6(9):e23486
pubmed: 21949680
PLoS One. 2015 Oct 13;10(10):e0140416
pubmed: 26462203
Malar J. 2015 Sep 22;14:363
pubmed: 26395166
Proc Natl Acad Sci U S A. 2015 Jun 2;112(22):7067-72
pubmed: 25941365
Malar J. 2015 Dec 10;13 Suppl 1:497
pubmed: 26651993
Emerg Infect Dis. 2020 May;26(5):902-909
pubmed: 32310062
J Infect Dis. 2015 Apr 1;211(7):1087-96
pubmed: 25336725
Am J Trop Med Hyg. 2017 Oct;97(4_Suppl):43-48
pubmed: 29064360
PLoS Genet. 2017 Oct 27;13(10):e1007065
pubmed: 29077712
Trends Genet. 2006 Aug;22(8):424-9
pubmed: 16806567
Am J Trop Med Hyg. 2016 Oct 5;95(4):811-816
pubmed: 27430541
BMC Med. 2018 Oct 18;16(1):190
pubmed: 30333020
Antimicrob Agents Chemother. 2011 Jan;55(1):155-64
pubmed: 20956597
Malar J. 2016 Jul 22;15(1):376
pubmed: 27443992
Malar J. 2008 Oct 29;7:223
pubmed: 18959790
Malar J. 2018 May 15;17(1):196
pubmed: 29764422
Mol Biol Evol. 2016 Jul;33(7):1870-4
pubmed: 27004904