Targeted gene disruption of ATP synthases 6-1 and 6-2 in the mitochondrial genome of Arabidopsis thaliana by mitoTALENs.
Arabidopsis
Mitochondrial genome editing
genome editing
mitochondrial genome
technical advance
Journal
The Plant journal : for cell and molecular biology
ISSN: 1365-313X
Titre abrégé: Plant J
Pays: England
ID NLM: 9207397
Informations de publication
Date de publication:
12 2020
12 2020
Historique:
received:
14
07
2020
revised:
22
09
2020
accepted:
13
10
2020
pubmed:
25
10
2020
medline:
11
5
2021
entrez:
24
10
2020
Statut:
ppublish
Résumé
We recently achieved targeted disruptions of cytoplasmic male sterility (CMS)-associated genes in the mitochondrial genomes of rice and rapeseed by using mitochondria-targeted transcription activator-like effector nucleases (mitoTALENs). It was the first report of stable and heritable targeted gene modification of plant mitochondrial genomes. Here, we attempted to use mitoTALENs to disrupt two mitochondrial genes in the model plant Arabidopsis thaliana(Arabidopsis) using three different promoters and two types of TALENs. The targets were the two isoforms of the ATP synthase subunit 6 gene, atp6-1 and atp6-2. Each of these genes was successfully deleted and the mitochondrial genomes were recovered in a homoplasmic state. The nuclear genome also has a copy of atp6-1, and we were able to confirm that it was the mitochondrial gene and not the nuclear pseudogene that was knocked out. Among the three mitoTALEN promoters tried, the RPS5A promoter was the most effective. Conventional mitoTALENs were more effective than single-molecule mito-compactTALENs. Targeted mitochondrial gene deletion was achieved by crossing as well as by floral-dip transformation to introduce the mitoTALEN constructs into the nucleus. The gene disruptions were caused by large (kb-size) deletions. The ends of the remaining sequences were connected to distant loci, mostly by illegitimate homologous recombinations between repeats.
Substances chimiques
Arabidopsis Proteins
0
Transcription Activator-Like Effector Nucleases
EC 3.1.-
ATP synthase subunit 6
EC 3.6.1.-
ATMG00410 protein, Arabidopsis
EC 3.6.3.-
ATMG01170 protein, Arabidopsis
EC 3.6.3.-
Mitochondrial Proton-Translocating ATPases
EC 3.6.3.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1459-1471Commentaires et corrections
Type : CommentIn
Informations de copyright
© 2020 Society for Experimental Biology and John Wiley & Sons Ltd.
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