Interhelical interactions within the STIM1 CC1 domain modulate CRAC channel activation.


Journal

Nature chemical biology
ISSN: 1552-4469
Titre abrégé: Nat Chem Biol
Pays: United States
ID NLM: 101231976

Informations de publication

Date de publication:
02 2021
Historique:
received: 16 04 2020
accepted: 11 09 2020
revised: 04 09 2020
pubmed: 28 10 2020
medline: 9 3 2021
entrez: 27 10 2020
Statut: ppublish

Résumé

The calcium release activated calcium channel is activated by the endoplasmic reticulum-resident calcium sensor protein STIM1. On activation, STIM1 C terminus changes from an inactive, tight to an active, extended conformation. A coiled-coil clamp involving the CC1 and CC3 domains is essential in controlling STIM1 activation, with CC1 as the key entity. The nuclear magnetic resonance-derived solution structure of the CC1 domain represents a three-helix bundle stabilized by interhelical contacts, which are absent in the Stormorken disease-related STIM1 R304W mutant. Two interhelical sites between the CC1α

Identifiants

pubmed: 33106661
doi: 10.1038/s41589-020-00672-8
pii: 10.1038/s41589-020-00672-8
pmc: PMC7610458
mid: EMS118356
doi:

Substances chimiques

Calcium Release Activated Calcium Channels 0
Neoplasm Proteins 0
ORAI1 Protein 0
ORAI1 protein, human 0
STIM1 protein, human 0
Stromal Interaction Molecule 1 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

196-204

Subventions

Organisme : Austrian Science Fund FWF
ID : W 1250
Pays : Austria

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Auteurs

Petr Rathner (P)

Institute of Organic Chemistry, Johannes Kepler University Linz, Linz, Austria.
Institute of Inorganic Chemistry, Johannes Kepler University Linz, Linz, Austria.

Marc Fahrner (M)

Institute of Biophysics, Johannes Kepler University Linz, Linz, Austria.

Linda Cerofolini (L)

Magnetic Resonance Center (CERM), University of Florence and Consorzio Interuniversitario Risonanze Magnetiche di Metallo Proteine (CIRMMP), Sesto Fiorentino, Italy.

Herwig Grabmayr (H)

Institute of Biophysics, Johannes Kepler University Linz, Linz, Austria.

Ferdinand Horvath (F)

Institute for Theoretical Physics, Johannes Kepler University Linz, Linz, Austria.

Heinrich Krobath (H)

Institute for Theoretical Physics, Johannes Kepler University Linz, Linz, Austria.

Agrim Gupta (A)

Institute of Organic Chemistry, Johannes Kepler University Linz, Linz, Austria.

Enrico Ravera (E)

Magnetic Resonance Center (CERM), University of Florence and Consorzio Interuniversitario Risonanze Magnetiche di Metallo Proteine (CIRMMP), Sesto Fiorentino, Italy.
Department of Chemistry, University of Florence, Sesto Fiorentino, Italy.

Marco Fragai (M)

Magnetic Resonance Center (CERM), University of Florence and Consorzio Interuniversitario Risonanze Magnetiche di Metallo Proteine (CIRMMP), Sesto Fiorentino, Italy.
Department of Chemistry, University of Florence, Sesto Fiorentino, Italy.

Matthias Bechmann (M)

Institute of Organic Chemistry, Johannes Kepler University Linz, Linz, Austria.

Thomas Renger (T)

Institute for Theoretical Physics, Johannes Kepler University Linz, Linz, Austria.

Claudio Luchinat (C)

Magnetic Resonance Center (CERM), University of Florence and Consorzio Interuniversitario Risonanze Magnetiche di Metallo Proteine (CIRMMP), Sesto Fiorentino, Italy.
Department of Chemistry, University of Florence, Sesto Fiorentino, Italy.

Christoph Romanin (C)

Institute of Biophysics, Johannes Kepler University Linz, Linz, Austria. christoph.romanin@jku.at.

Norbert Müller (N)

Institute of Organic Chemistry, Johannes Kepler University Linz, Linz, Austria. norbert.mueller@jku.at.
Faculty of Science, University of South Bohemia, České Budějovice, Czech Republic. norbert.mueller@jku.at.

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Classifications MeSH