Function of a viral genome packaging motor from bacteriophage T4 is insensitive to DNA sequence.
Journal
Nucleic acids research
ISSN: 1362-4962
Titre abrégé: Nucleic Acids Res
Pays: England
ID NLM: 0411011
Informations de publication
Date de publication:
18 11 2020
18 11 2020
Historique:
accepted:
29
09
2020
revised:
12
09
2020
received:
19
05
2020
pubmed:
30
10
2020
medline:
4
2
2021
entrez:
29
10
2020
Statut:
ppublish
Résumé
Many viruses employ ATP-powered motors during assembly to translocate DNA into procapsid shells. Previous reports raise the question if motor function is modulated by substrate DNA sequence: (i) the phage T4 motor exhibits large translocation rate fluctuations and pauses and slips; (ii) evidence suggests that the phage phi29 motor contacts DNA bases during translocation; and (iii) one theoretical model, the 'B-A scrunchworm', predicts that 'A-philic' sequences that transition more easily to A-form would alter motor function. Here, we use single-molecule optical tweezers measurements to compare translocation of phage, plasmid, and synthetic A-philic, GC rich sequences by the T4 motor. We observed no significant differences in motor velocities, even with A-philic sequences predicted to show higher translocation rate at high applied force. We also observed no significant changes in motor pausing and only modest changes in slipping. To more generally test for sequence dependence, we conducted correlation analyses across pairs of packaging events. No significant correlations in packaging rate, pausing or slipping versus sequence position were detected across repeated measurements with several different DNA sequences. These studies suggest that viral genome packaging is insensitive to DNA sequence and fluctuations in packaging motor velocity, pausing and slipping are primarily stochastic temporal events.
Identifiants
pubmed: 33119757
pii: 5943198
doi: 10.1093/nar/gkaa875
pmc: PMC7672480
doi:
Substances chimiques
DNA, Viral
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
11602-11614Subventions
Organisme : NIAID NIH HHS
ID : R56 AI081726
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM088186
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM118817
Pays : United States
Organisme : NIAID NIH HHS
ID : U01 AI082086
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI081726
Pays : United States
Informations de copyright
© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.
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