Short tandem repeat profiling for the authentication of cancer stem-like cells.


Journal

International journal of cancer
ISSN: 1097-0215
Titre abrégé: Int J Cancer
Pays: United States
ID NLM: 0042124

Informations de publication

Date de publication:
15 03 2021
Historique:
received: 14 02 2020
revised: 09 10 2020
accepted: 12 10 2020
pubmed: 1 11 2020
medline: 20 7 2021
entrez: 31 10 2020
Statut: ppublish

Résumé

Colorectal and glioblastoma cancer stem-like cells (CSCs) are essential for translational research. Cell line authentication by short tandem repeat (STR) profiling ensures reproducibility of results in oncology research. This technique enables to identify mislabeling or cross-contamination of cell lines. In our study, we provide a reference dataset for a panel of colorectal and glioblastoma CSCs that allows authentication. Each cell line was entered into the cell Line Integrated Molecular Authentication database 2.1 to be compared to the STR profiles of 4485 tumor cell lines. This article also provides clinical data of patients from whom CSCs arose and data on the parent tumor stage and mutations. STR profiles and information of our CSCs are also available in the Cellosaurus database (ExPASy) as identified by unique research resource identifier codes.

Identifiants

pubmed: 33128777
doi: 10.1002/ijc.33370
pmc: PMC7894552
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

1489-1498

Informations de copyright

© 2020 The Authors. International Journal of Cancer published by John Wiley & Sons Ltd on behalf of Union for International Cancer Control.

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Auteurs

Paola Visconti (P)

IRCCS Ospedale Policlinico San Martino, Interlab Cell Line Collection (ICLC), Biological Resource Center (CRB-HSM), Genoa, Italy.

Federica Parodi (F)

IRCCS Ospedale Policlinico San Martino, Interlab Cell Line Collection (ICLC), Biological Resource Center (CRB-HSM), Genoa, Italy.

Barbara Parodi (B)

IRCCS Ospedale Policlinico San Martino, Interlab Cell Line Collection (ICLC), Biological Resource Center (CRB-HSM), Genoa, Italy.

Lucia Casarino (L)

Department of Legal and Forensic Medicine, University of Genoa, Genoa, Italy.

Paolo Romano (P)

IRCCS Ospedale Policlinico San Martino, Proteomics Service, Scientific Direction, Genoa, Italy.

Mariachiara Buccarelli (M)

Department of Oncology and Molecular Medicine, Istituto Superiore di Sanità, Rome, Italy.

Roberto Pallini (R)

Fondazione Policlinico Universitario A. Gemelli IRCCS - Institute of Neurosurgery, Catholic University School of Medicine, Rome, Italy.

Quintino Giorgio D'Alessandris (QG)

Fondazione Policlinico Universitario A. Gemelli IRCCS - Institute of Neurosurgery, Catholic University School of Medicine, Rome, Italy.

Andrea Montori (A)

Department of Clinical and Molecular Medicine, Sant'Andrea Hospital, University "La Sapienza", Rome, Italy.

Emanuela Pilozzi (E)

Department of Clinical and Molecular Medicine, Sant'Andrea Hospital, University "La Sapienza", Rome, Italy.

Lucia Ricci-Vitiani (L)

Department of Oncology and Molecular Medicine, Istituto Superiore di Sanità, Rome, Italy.

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Classifications MeSH