Harnessing DNA for nanothermometry.
DNA
Hoechst
anisotropy
nanothermometers
temperature
thermal information
Journal
Journal of biophotonics
ISSN: 1864-0648
Titre abrégé: J Biophotonics
Pays: Germany
ID NLM: 101318567
Informations de publication
Date de publication:
02 2021
02 2021
Historique:
received:
26
08
2020
revised:
05
10
2020
accepted:
20
10
2020
pubmed:
1
11
2020
medline:
13
7
2021
entrez:
31
10
2020
Statut:
ppublish
Résumé
Temperature measurement at the nanoscale has brought insight to a wide array of research interests in modern chemistry, physics, and biology. These measurements have been enabled by the advent of nanothermometers, which relay nanoscale temperature information through the analysis of their intrinsic photophysical behavior. In the past decade, several nanothermometers have been developed including dyes, nanodiamonds, fluorescent proteins, nucleotides, and nanoparticles. However, temperature measurement using intact DNA has not yet been achieved. Here, we present a method to study the temperature sensitivity of the DNA molecule within a physiologic temperature range when complexed with fluorescent dye. We theoretically and experimentally report the temperature sensitivity of the DNA-Hoechst 33342 complex in different sizes of double-stranded oligonucleotides and plasmids, showing its potential use as a nanothermometer. These findings allow for extending the thermal study of DNA to several research fields including DNA nanotechnology, optical tweezers, and DNA nanoparticles.
Identifiants
pubmed: 33128802
doi: 10.1002/jbio.202000341
pmc: PMC8009499
mid: NIHMS1679403
doi:
Substances chimiques
Fluorescent Dyes
0
Nanodiamonds
0
DNA
9007-49-2
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e202000341Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM124133
Pays : United States
Organisme : NIH HHS
ID : R01GM124133
Pays : United States
Informations de copyright
© 2020 Wiley-VCH GmbH.
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