Amelioration of airway inflammation and pulmonary edema by Teucrium stocksianum via attenuation of pro-inflammatory cytokines and up-regulation of AQP1 and AQP5.
Animals
Aquaporin 1
/ drug effects
Aquaporin 5
/ drug effects
Asthma
/ drug therapy
Disease Models, Animal
Female
Hypersensitivity
/ drug therapy
Immunologic Factors
/ administration & dosage
Inflammation
/ drug therapy
Interleukin-4
Interleukin-5
Male
Mice
Mice, Inbred BALB C
Plant Extracts
/ administration & dosage
Pulmonary Edema
/ drug therapy
Respiratory Tract Diseases
/ drug therapy
Teucrium
Interleukin-5
airway inflammation
aquaporins
interleukin-4
pulmonary edema
Journal
Respiratory physiology & neurobiology
ISSN: 1878-1519
Titre abrégé: Respir Physiol Neurobiol
Pays: Netherlands
ID NLM: 101140022
Informations de publication
Date de publication:
02 2021
02 2021
Historique:
received:
11
08
2020
revised:
17
10
2020
accepted:
25
10
2020
pubmed:
5
11
2020
medline:
25
11
2021
entrez:
4
11
2020
Statut:
ppublish
Résumé
Current study investigates the immunomodulatory effects of T. stocksianum using mouse model of ovalbumin (OVA)-induced allergic asthma. The mice were treated with methanolic extract, n-hexane, and ethyl acetate fractions for consecutive 7 days along with intranasal challenge. The mRNA expression levels of interleukin-4 (IL-4), IL-5, Aquaporin-1 (AQP1) and Aquaporin-5 (AQP5) were evaluated using reverse transcription polymerase chain reaction. The data showed that T. stocksianum significantly reduced airway inflammation as indicated by reduced inflammatory cell infiltration in lungs, and attenuated total and differential leukocyte counts both in blood and BALF. Expression levels of pro-inflammatory IL-4 and IL-5 in lungs were also found significantly reduced. T. stocksianum significantly reduced pulmonary edema as indicated by reduced lung wet/dry ratio and goblet cell hyperplasia. AQP1 and AQP5 expression levels were also found elevated in treatment groups. In conclusion, T. stocksianum possesses anti-asthmatic activity which may be attributed to reduction in IL-4 and IL-5 expression levels, and elevation in AQP1 and AQP5 expression levels.
Identifiants
pubmed: 33144273
pii: S1569-9048(20)30227-5
doi: 10.1016/j.resp.2020.103569
pii:
doi:
Substances chimiques
Aqp1 protein, mouse
0
Aqp5 protein, mouse
0
Aquaporin 5
0
Il4 protein, mouse
0
Immunologic Factors
0
Interleukin-5
0
Plant Extracts
0
Aquaporin 1
146410-94-8
Interleukin-4
207137-56-2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
103569Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.