Genetic profiling of protein burden and nuclear export overload.
Active Transport, Cell Nucleus
/ genetics
Cell Nucleus
/ metabolism
Genetic Profile
Genomics
Green Fluorescent Proteins
Mutation
Nuclear Export Signals
/ genetics
Proteasome Endopeptidase Complex
Protein Biosynthesis
/ genetics
Saccharomyces cerevisiae
/ genetics
Saccharomyces cerevisiae Proteins
/ genetics
S. cerevisiae
genetic profiling
genetics
genomics
nuclear export
overexpression
protein burden
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
04 11 2020
04 11 2020
Historique:
received:
02
12
2019
accepted:
01
11
2020
pubmed:
5
11
2020
medline:
24
3
2021
entrez:
4
11
2020
Statut:
epublish
Résumé
Overproduction (op) of proteins triggers cellular defects. One of the consequences of overproduction is the protein burden/cost, which is produced by an overloading of the protein synthesis process. However, the physiology of cells under a protein burden is not well characterized. We performed genetic profiling of protein burden by systematic analysis of genetic interactions between GFP-op, surveying both deletion and temperature-sensitive mutants in budding yeast. We also performed genetic profiling in cells with overproduction of triple-GFP (tGFP), and the nuclear export signal-containing tGFP (NES-tGFP). The mutants specifically interacted with GFP-op were suggestive of unexpected connections between actin-related processes like polarization and the protein burden, which was supported by morphological analysis. The tGFP-op interactions suggested that this protein probe overloads the proteasome, whereas those that interacted with NES-tGFP involved genes encoding components of the nuclear export process, providing a resource for further analysis of the protein burden and nuclear export overload.
Identifiants
pubmed: 33146608
doi: 10.7554/eLife.54080
pii: 54080
pmc: PMC7673788
doi:
pii:
Substances chimiques
Nuclear Export Signals
0
Saccharomyces cerevisiae Proteins
0
Green Fluorescent Proteins
147336-22-9
Proteasome Endopeptidase Complex
EC 3.4.25.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Japan Society for the Promotion of Science
ID : 17H03618
Pays : International
Organisme : Japan Society for the Promotion of Science
ID : 15KK0258
Pays : International
Organisme : Japan Society for the Promotion of Science
ID : 18K19300
Pays : International
Organisme : Japan Society for the Promotion of Science
ID : 20H03242
Pays : International
Organisme : Japan Society for the Promotion of Science
ID : 19H03205
Pays : International
Informations de copyright
© 2020, Kintaka et al.
Déclaration de conflit d'intérêts
RK, KM, SN, HK, KK, SO, YO, BA, CB, HM No competing interests declared
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