Lipase@zeolitic imidazolate framework ZIF-90: A highly stable and recyclable biocatalyst for the synthesis of fruity banana flavour.
Esterification
Immobilization
Isoamyl butyrate
Porcine pancreatic lipase
Zeolitic imidazolate framework
Journal
International journal of biological macromolecules
ISSN: 1879-0003
Titre abrégé: Int J Biol Macromol
Pays: Netherlands
ID NLM: 7909578
Informations de publication
Date de publication:
01 Jan 2021
01 Jan 2021
Historique:
received:
07
06
2020
revised:
02
11
2020
accepted:
03
11
2020
pubmed:
9
11
2020
medline:
10
4
2021
entrez:
8
11
2020
Statut:
ppublish
Résumé
A zeolitic imidazolate framework (ZIF-90) has been synthesized through solvothermal method. The structure was characterized by means of FT-IR spectroscopy, X-ray diffraction, thermogravimetric analysis (TGA), and scanning electron microscopy (SEM)/energy dispersive X-ray spectroscopy (EDS). The synthesized ZIF-90 was applied as a support for immobilization of porcine pancreatic lipase (PPL). The immobilized enzyme (PPL@ZIF-90) exhibited immobilization yield and efficiency of 66 ± 1.8% and 89 ± 1.4%, respectively. The pH and thermal stability of PPL was improved after immobilization and the initial activity was retained at about 57% after 20 days of storage at 4 °C for PPL@ZIF-90. Moreover, about 57% of the original activity was remained following 10 cycles of application. In Michaelis-Menten kinetic studies, K
Identifiants
pubmed: 33161085
pii: S0141-8130(20)34936-9
doi: 10.1016/j.ijbiomac.2020.11.011
pii:
doi:
Substances chimiques
Butyrates
0
Enzymes, Immobilized
0
Flavoring Agents
0
Imidazoles
0
Metal-Organic Frameworks
0
Lipase
EC 3.1.1.3
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1301-1311Informations de copyright
Copyright © 2020. Published by Elsevier B.V.
Déclaration de conflit d'intérêts
Declaration of competing interest None.