CEP164C regulates flagellum length in stable flagella.
Journal
The Journal of cell biology
ISSN: 1540-8140
Titre abrégé: J Cell Biol
Pays: United States
ID NLM: 0375356
Informations de publication
Date de publication:
04 01 2021
04 01 2021
Historique:
received:
29
01
2020
revised:
11
08
2020
accepted:
20
10
2020
entrez:
9
11
2020
pubmed:
10
11
2020
medline:
19
5
2021
Statut:
ppublish
Résumé
Cilia and flagella are required for cell motility and sensing the external environment and can vary in both length and stability. Stable flagella maintain their length without shortening and lengthening and are proposed to "lock" at the end of growth, but molecular mechanisms for this lock are unknown. We show that CEP164C contributes to the locking mechanism at the base of the flagellum in Trypanosoma brucei. CEP164C localizes to mature basal bodies of fully assembled old flagella, but not to growing new flagella, and basal bodies only acquire CEP164C in the third cell cycle after initial assembly. Depletion of CEP164C leads to dysregulation of flagellum growth, with continued growth of the old flagellum, consistent with defects in a flagellum locking mechanism. Inhibiting cytokinesis results in CEP164C acquisition on the new flagellum once it reaches the old flagellum length. These results provide the first insight into the molecular mechanisms regulating flagella growth in cells that must maintain existing flagella while growing new flagella.
Identifiants
pubmed: 33165561
pii: 211523
doi: 10.1083/jcb.202001160
pmc: PMC7833213
pii:
doi:
Substances chimiques
Protozoan Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/M000532/1
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 104627/Z/14/Z
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 108445\/Z\/15\/Z
Pays : United Kingdom
Informations de copyright
© 2020 Atkins et al.
Références
Curr Biol. 2018 Dec 3;28(23):3802-3814.e3
pubmed: 30449671
Open Biol. 2012 Feb;2(2):110037
pubmed: 22645659
J Cell Sci. 2006 May 1;119(Pt 9):1852-63
pubmed: 16608878
J Cell Biol. 2015 Jan 19;208(2):223-37
pubmed: 25583998
Curr Biol. 2012 May 8;22(9):R330-9
pubmed: 22575475
J Biol Chem. 2015 May 15;290(20):12765-78
pubmed: 25825494
PLoS Genet. 2014 Oct 23;10(10):e1004594
pubmed: 25340510
Cell Motil Cytoskeleton. 2009 Jan;66(1):24-35
pubmed: 19009637
Mol Biol Cell. 2005 Jan;16(1):270-8
pubmed: 15496456
J Cell Biol. 1982 Jun;93(3):576-82
pubmed: 6811596
Acta Trop. 1979 Sep;36(3):289-92
pubmed: 43092
Mol Cells. 2017 Apr;40(4):243-253
pubmed: 28401750
Int Rev Cell Mol Biol. 2013;303:101-38
pubmed: 23445809
J Cell Sci. 2010 May 1;123(Pt 9):1407-13
pubmed: 20388734
Traffic. 2007 Oct;8(10):1323-30
pubmed: 17645436
J Cell Biol. 2007 Oct 22;179(2):321-30
pubmed: 17954613
Mol Biochem Parasitol. 1996 May;77(2):235-9
pubmed: 8813669
Cell Microbiol. 2020 Mar;22(3):e13162
pubmed: 31945244
Nature. 1998 Feb 5;391(6667):548
pubmed: 9468133
J Cell Biol. 2014 Oct 13;207(1):123-37
pubmed: 25313408
J Eukaryot Microbiol. 1999 Mar-Apr;46(2):105-9
pubmed: 10361731
J Biol Chem. 2005 Apr 8;280(14):14085-96
pubmed: 15653691
Mol Biol Cell. 2015 Dec 1;26(24):4358-72
pubmed: 26424803
J Cell Biol. 2012 Dec 24;199(7):1083-101
pubmed: 23253480
Trends Parasitol. 2017 Feb;33(2):80-82
pubmed: 27863903
Int Rev Cytol. 2005;244:227-85
pubmed: 16157182
mBio. 2017 Jan 3;8(1):
pubmed: 28049148
J Cell Biol. 1987 Mar;104(3):431-8
pubmed: 3818788
J Cell Biol. 2001 Oct 29;155(3):405-14
pubmed: 11684707
Mol Biochem Parasitol. 2003 Apr 25;128(1):115-8
pubmed: 12706807
Open Biol. 2015 Jan;5(1):140197
pubmed: 25567099
Proc Natl Acad Sci U S A. 2014 Jul 15;111(28):E2841-50
pubmed: 24982133