miR-19a mitigates hypoxia/reoxygenation-induced injury by depressing CCL20 and inactivating MAPK pathway in human embryonic cardiomyocytes.
Apoptosis
/ genetics
Cell Hypoxia
/ genetics
Cell Survival
/ genetics
Chemokine CCL20
/ genetics
Gene Expression Regulation
/ genetics
Humans
MicroRNAs
/ genetics
Mitogen-Activated Protein Kinase Kinases
/ genetics
Molecular Targeted Therapy
Myocardial Infarction
/ genetics
Myocardial Reperfusion Injury
/ genetics
Myocytes, Cardiac
/ metabolism
Oxygen
/ metabolism
Signal Transduction
/ genetics
CCL20
H/R
MAPK pathway
Myocardial infarction
miR-19a
Journal
Biotechnology letters
ISSN: 1573-6776
Titre abrégé: Biotechnol Lett
Pays: Netherlands
ID NLM: 8008051
Informations de publication
Date de publication:
Feb 2021
Feb 2021
Historique:
received:
18
06
2020
accepted:
31
10
2020
pubmed:
10
11
2020
medline:
11
9
2021
entrez:
9
11
2020
Statut:
ppublish
Résumé
Myocardial infarction (MI) is a prevalent cardiovascular puzzle and a mainspring of disease-induced mortality. We performed this investigation to detect the role of putative important miRNAs or genes in MI. CCL20 may be a potential therapeutic target, which was directly targeted and negatively regulated by miR-19a. CCL20 expression was significantly increased in MI tissue samples, but miR-19a was expressed at lower levels in MI. H/R treatment inhibited cell viability and induced an increase of apoptotic rate compared with Sham group. However, miR-19a mimic relieved the H/R-stimulated injury to cardiomyocytes. Protective effect of miR-19a against H/R in cardiomyocytes was reversed by CCL20 enhancement, and MAPK pathway was inactivated during this progression. miR-19a eliminates the H/R-induced injury in cardiomyocytes through directly targeting CCL20 and attenuating the activity of MAPK signaling pathway. These observations highlighted the therapeutic roles of miR-19a and CCL20 for MI treatment.
Identifiants
pubmed: 33165673
doi: 10.1007/s10529-020-03045-2
pii: 10.1007/s10529-020-03045-2
doi:
Substances chimiques
CCL20 protein, human
0
Chemokine CCL20
0
MIRN19 microRNA, human
0
MicroRNAs
0
Mitogen-Activated Protein Kinase Kinases
EC 2.7.12.2
Oxygen
S88TT14065
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
393-405Références
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