Transcriptomics in Trichoderma reesei.
DNA, Complementary
/ genetics
DNA, Fungal
/ genetics
Data Analysis
Gene Expression Profiling
/ methods
Gene Expression Regulation, Fungal
Gene Library
Hypocreales
/ genetics
Principal Component Analysis
RNA, Fungal
/ genetics
RNA, Messenger
/ genetics
Reproducibility of Results
Transcriptome
/ genetics
Big data
Gene expression
RNA-seq
Transcriptome
Trichoderma reesei
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2021
2021
Historique:
entrez:
9
11
2020
pubmed:
10
11
2020
medline:
25
3
2021
Statut:
ppublish
Résumé
Transcriptomics is a powerful technique to study gene expression. The main purpose of transcriptome studies in the filamentous fungus Trichoderma reesei is the analysis of differentially expressed genes as a transcriptional response of the genome to different environmental stimuli or physiological conditions such as sugar availability, nitrogen metabolism, pH response, and oxidative stress, among others. Here we describe the full protocol of RNA sequencing methodology from RNA isolation to data analysis in order to access the T. reesei transcriptome.
Identifiants
pubmed: 33165792
doi: 10.1007/978-1-0716-1048-0_18
doi:
Substances chimiques
DNA, Complementary
0
DNA, Fungal
0
RNA, Fungal
0
RNA, Messenger
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM