Reciprocal growth control by competitive binding of nucleotide second messengers to a metabolic switch in Caulobacter crescentus.
Binding Sites
/ physiology
Binding, Competitive
/ physiology
Caulobacter crescentus
/ genetics
Cyclic GMP
/ analogs & derivatives
Gene Expression Regulation, Bacterial
/ genetics
Glucose
/ metabolism
Guanosine Pentaphosphate
/ metabolism
Oxidation-Reduction
Second Messenger Systems
/ genetics
Signal Transduction
/ genetics
Transferases
/ metabolism
Journal
Nature microbiology
ISSN: 2058-5276
Titre abrégé: Nat Microbiol
Pays: England
ID NLM: 101674869
Informations de publication
Date de publication:
01 2021
01 2021
Historique:
received:
11
12
2019
accepted:
02
10
2020
pubmed:
11
11
2020
medline:
17
3
2021
entrez:
10
11
2020
Statut:
ppublish
Résumé
Bacteria use small signalling molecules such as (p)ppGpp or c-di-GMP to tune their physiology in response to environmental changes. It remains unclear whether these regulatory networks operate independently or whether they interact to optimize bacterial growth and survival. We report that (p)ppGpp and c-di-GMP reciprocally regulate the growth of Caulobacter crescentus by converging on a single small-molecule-binding protein, SmbA. While c-di-GMP binding inhibits SmbA, (p)ppGpp competes for the same binding site to sustain SmbA activity. We demonstrate that (p)ppGpp specifically promotes Caulobacter growth on glucose, whereas c-di-GMP inhibits glucose consumption. We find that SmbA contributes to this metabolic switch and promotes growth on glucose by quenching the associated redox stress. The identification of an effector protein that acts as a central regulatory hub for two global second messengers opens up future studies on specific crosstalk between small-molecule-based regulatory networks.
Identifiants
pubmed: 33168988
doi: 10.1038/s41564-020-00809-4
pii: 10.1038/s41564-020-00809-4
doi:
Substances chimiques
Guanosine Pentaphosphate
38918-96-6
bis(3',5')-cyclic diguanylic acid
61093-23-0
Transferases
EC 2.-
Cyclic GMP
H2D2X058MU
Glucose
IY9XDZ35W2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
59-72Commentaires et corrections
Type : CommentIn
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