Semicontinuous system for the production of recombinant mCherry protein in Chlamydomonas reinhardtii.
biotechnology
bubble column photobioreactor
heterologous protein
microalgae cultivation
Journal
Biotechnology progress
ISSN: 1520-6033
Titre abrégé: Biotechnol Prog
Pays: United States
ID NLM: 8506292
Informations de publication
Date de publication:
03 2021
03 2021
Historique:
revised:
23
10
2020
received:
05
08
2020
accepted:
28
10
2020
pubmed:
11
11
2020
medline:
29
1
2022
entrez:
10
11
2020
Statut:
ppublish
Résumé
Biotechnology advances have allowed bacteria, yeasts, plants, mammalian and insect cells to function as heterologous protein expression systems. Recently, microalgae have gained attention as an innovative platform for recombinant protein production, due to low culture media cost, compared to traditional systems, as well as the fact that microalgae such as Chlamydomonas reinhardtii are considered safe (GRAS) by the Food and Drug Administration (FDA). Previous studies showed that recombinant protein production in traditional platforms by semicontinuous process increased biomass and bio product productivity, when compared to batch process. As there is a lack of studies on semicontinuous process for recombinant protein production in microalgae, the production of recombinant mCherry fluorescent protein was evaluated by semicontinuous cultivation of Chlamydomonas reinhardtii in bubble column photobioreactor. This semicontinuous cultivation process was evaluated in the following conditions: 20%, 40%, and 60% culture portion withdrawal. The highest culture withdrawal percentage (60%) provided the best results, as an up to 161% increase in mCherry productivity (454.5 RFU h
Substances chimiques
Culture Media
0
Luminescent Agents
0
Luminescent Proteins
0
Recombinant Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e3101Subventions
Organisme : Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
ID : 001
Organisme : Fundação de Amparo à Pesquisa do Estado de São Paulo
ID : 2016/12992-6
Organisme : Fundação de Amparo à Pesquisa do Estado de São Paulo
ID : 2017/24486-0
Organisme : FDA HHS
Pays : United States
Organisme : FDA HHS
Pays : United States
Informations de copyright
© 2020 American Institute of Chemical Engineers.
Références
Wijffels RH. Potential of sponges and microalgae for marine biotechnology. Trends Biotechnol. 2008;26:26-31. https://doi.org/10.1016/j.tibtech.2007.10.002.
Walker TL, Purton S, Becker DK, Collet C. Microalgae as bioreactors. Plant Cell Rep. 2005;24(11):629-641. https://doi.org/10.1007/s00299-005-0004-6.
Norouzi M, Pickford AR, Butt LE, Vincent HA, Callaghan AJ. Application of mRNA arrays for the production of mCherry reporter-protein arrays for quantitative gene expression analysis. ACS Synth Biol. 2019;8:207-215. https://doi.org/10.1021/acssynbio.8b00266.
Gonzales, CD. Construcción y expresión de una proteína de fusión C-terminal entre la cadena kappa del anticuerpo 14D9 y una proteína fluorescente roja. BA Thesis. Argentina: Universidad Nacional de La Plata. 2010.
Rasala BA, Barrera DJ, Ng J, et al. Expanding the spectral palette of fluorescent proteins for the green microalga Chlamydomonas reinhardtii. Plant J. 2013;74(4):545-556. https://doi.org/10.1111/tpj.12165.
Molino JVD, de CJCM, Mayfield SP. Comparison of secretory signal peptides for heterologous protein expression in microalgae: expanding the secretion portfolio for Chlamydomonas reinhardtii. PLoS One. 2018;13(2):1-20. https://doi.org/10.1371/journal.pone.0192433.
Hui CY, Guo Y, Zhang W, Huang XQ. Rapid monitoring of the target protein expression with a fluorescent signal based on a dicistronic construct in Escherichia coli. AMB Express. 2018;8(1):81. https://doi.org/10.1186/s13568-018-0612-5.
Cheong DE, Ko KC, Han Y, et al. Enhancing functional expression of heterologous proteins through random substitution of genetic codes in the 5′ coding region. Biotechnol Bioeng. 2015;112(4):822-826. https://doi.org/10.1002/bit.25478.
Lee S, Lim WA, Thorn KS. Improved blue, green, and red fluorescent protein tagging vectors for S. cerevisiae. PLoS One. 2013;8(7):e67902. https://doi.org/10.1371/journal.pone.0067902.
Shaner NC, Lin MZ, McKeown MR, et al. Evaluating and improving the photostability of fluorescent proteins. Proc SPIE. 2009;7191:719105. https://doi.org/10.1117/12.814684.
Doherty GP, Bailey K, Lewis PJ. Stage-specific fluorescence intensity of GFP and mCherry during sporulation in Bacillus subtilis. BMC Res Notes. 2010;3:1-8. https://doi.org/10.1186/1756-0500-3-303.
Esland L, Larrea-Alvarez M, Purton S. Selectable markers and reporter genes for engineering the chloroplast of Chlamydomonas reinhardtii. Biology (Basel). 2018;7(46):1-26. https://doi.org/10.3390/biology7040046.
Carvalho JCM, Matsudo MC, Bezerra RP, Ferreira-Camargo LS, Sato S. Microalgae bioreactors. In: Bajpai R, Prokop A, Zappi M, eds. Algal Biorefineries. Dordrecht: Springer Science + Business Media Dordrecht; 2014. https://doi.org/10.1007/978-94-007-7494-0_4.
Schmideder A, Weuster-Botz D. High-performance recombinant protein production with Escherichia coli in continuously operated cascades of stirred-tank reactors. J Ind Microbiol Biotechnol. 2017;44:1021-1029. https://doi.org/10.1007/s10295-017-1927-y.
Carvalho JCM, Bezerra RP, Matsudo MC, Sato S. Cultivation of Arthrospira (Spirulina) platensis by fed-batch process. In: Lee J, ed. Advanced Biofuels and Bioproducts. New York: Springer; 2013:781-805.
Bresaola MD, Morocho-Jácome AL, Matsudo MC, de Carvalho JCM. Semi-continuous process as a promising technique in Ankistrodesmus braunii cultivation in photobioreactor. J Appl Phycol. 2019;31(4):2197-2205. https://doi.org/10.1007/s10811-019-01774-0.
Reichert CC, Reinehr CO, Costa JAV. Semicontinuous cultivation of the cyanobacterium Spirulina platensis in a closed photobioreactor. Braz J Chem Eng. 2006;23(1):23-28. https://doi.org/10.1590/S0104-66322006000100003.
Lívanský K. Semicontinuous cultivation of autotrophic algae. Folia Microbiol (Praha). 1979;24(4):346-351. https://doi.org/10.1007/BF02926655.
Oncel S, Vardar-Sukan F. Photo-bioproduction of hydrogen by Chlamydomonas reinhardtii using a semi-continuous process regime. Int J Hydrogen Energy. 2009;34:7592-7602. https://doi.org/10.1016/j.ijhydene.2009.07.027.
Gorman DS, Levine RP. Cytochrome f and plastocyanin: their sequence in the photosynthetic electron transport chain of Chlamydomonas reinhardii. Proc Natl Acad Sci U S A. 1965;54(6):1665-1669. http://www.ncbi.nlm.nih.gov/pubmed/4379719.
Chlamydomonas Resource Center. Tap and Tris minimal. Accessed October 21, 2020. https://www.chlamycollection.org/methods/media-recipes/tap-and-tris-minimal/
Hutner SH, Provasoli L, Schatz AHC. Some approaches to the study of the role of metals in the metabolism of microorganisms. Proc Amer Philos Soc. 1950;94:152-170.
Diaz Arias, CA. Production processes of heterologous mCherry protein by Chlamydomonas reinhardtii. PhD Thesis. Brazil: Universidade de Sao Paulo. 2017.
Boonma S, Takarada T, Peerapornpisal Y, Pumas C, Chaiklangmuang S. Semi-continuous cultivation of microalgal consortium using low CO2 concentration for large-scale biofuel production. J Biotech Res. 2019;10:19-28.
Matsudo MC, Bezerra RP, Sato S, Perego P, Converti A, Carvalho JCM. Repeated fed-batch cultivation of Arthrospira (Spirulina) platensis using urea as nitrogen source. Biochem Eng J. 2009;43:52-57. https://doi.org/10.1016/j.bej.2008.08.009.
Zedler JA, Gangl D, Guerra T, Santos E, Verdelho VV, Robinson C. Pilot-scale cultivation of wall-deficient transgenic Chlamydomonas reinhardtii strains expressing recombinant proteins in the chloroplast. Appl Microbiol Biotechnol. 2016;100:7061-7070. https://doi.org/10.1007/s00253-016-7430-y10.1007/s00253-016-7430-y.
Weiner I, Atar S, Schweitzer S, et al. Enhancing heterologous expression in Chlamydomonas reinhardtii by transcript sequence optimization. Plant J. 2018;94:22-31. https://doi.org/10.1111/tpj.13836.
Lauersen KJ, Huber I, Wichmann J, et al. Investigating the dynamics of recombinant protein secretion from a microalgal host. J Biotechnol. 2015;215:62-71. https://doi.org/10.1016/j.jbiotec.2015.05.001.
Ghosh A, Sarkar S, Gayen K, Bhowmick TK. Effects of carbon, nitrogen, and phosphorus supplements on growth and biochemical composition of Podohedriella sp. (MCC44) isolated from Northeast India. Environ Prog Sustain Energy. 2020;39:1-12. https://doi.org/10.1002/ep.13378.
Michelon W, Da Silva MLB, Mezzari MP, Pirolli M, Prandini JM, Soares HM. Effects of nitrogen and phosphorus on biochemical composition of microalgae polyculture harvested from phycoremediation of piggery wastewater digestate. Appl Biochem Biotechnol 2016;178:1407-1419. https://doi.org/10.1007/s12010-015-1955-x.
Sousa AER, Nunes IVO, Muniz-Junior AB, Carvalho JCM, Da Silva LCM, Matsudo MC. Nitrogen supplementation for the production of Chlorella vulgaris biomass in secondary effluent from dairy industry. Biochem Eng J. 2021;165. https://doi.org/10.1016/j.bej.2020.107818.
Lauersen KJ, Vanderveer TL, Berger H, et al. Ice recrystallization inhibition mediated by a nuclear-expressed and -secreted recombinant ice-binding protein in the microalga Chlamydomonas reinhardtii. Appl Microbiol Biotechnol. 2013;97(22):9763-9772. https://doi.org/10.1007/s00253-013-5226-x.