A novel mechanism of β-glucosidase stimulation through a monosaccharide binding-induced conformational change.
Bioeconomy
GH1
Molecular dynamics simulation
Thermotoga petrophila
β-Glucosidases
Journal
International journal of biological macromolecules
ISSN: 1879-0003
Titre abrégé: Int J Biol Macromol
Pays: Netherlands
ID NLM: 7909578
Informations de publication
Date de publication:
01 Jan 2021
01 Jan 2021
Historique:
received:
13
03
2020
revised:
21
08
2020
accepted:
01
11
2020
pubmed:
13
11
2020
medline:
10
4
2021
entrez:
12
11
2020
Statut:
ppublish
Résumé
It is urgent the transition from a fossil fuel-based economy to a sustainable bioeconomy based on bioconversion technologies using renewable plant biomass feedstocks to produce high chemicals, bioplastics, and biofuels. β-Glucosidases are key enzymes responsible for degrading the plant cell wall polymers, as they cleave glucan-based oligo- and polysaccharides to generate glucose. Monosaccharide-tolerant or -stimulated β-glucosidases have been reported in the past decade. Here, we describe a novel mechanism of β-glucosidase stimulation by glucose and xylose. The glycoside hydrolase 1 family β-glucosidase from Thermotoga petrophila (TpBgl1) displays a typical glucose stimulation mechanism based on an increased V
Identifiants
pubmed: 33181222
pii: S0141-8130(20)34926-6
doi: 10.1016/j.ijbiomac.2020.11.001
pii:
doi:
Substances chimiques
Monosaccharides
0
Mutant Proteins
0
Xylose
A1TA934AKO
beta-Glucosidase
EC 3.2.1.21
Glucose
IY9XDZ35W2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1188-1196Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare no conflict of interests.