Atypical chemoreceptor arrays accommodate high membrane curvature.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
13 11 2020
13 11 2020
Historique:
received:
04
04
2020
accepted:
23
10
2020
entrez:
14
11
2020
pubmed:
15
11
2020
medline:
25
11
2020
Statut:
epublish
Résumé
The prokaryotic chemotaxis system is arguably the best-understood signaling pathway in biology. In all previously described species, chemoreceptors organize into a hexagonal (P6 symmetry) extended array. Here, we report an alternative symmetry (P2) of the chemotaxis apparatus that emerges from a strict linear organization of the histidine kinase CheA in Treponema denticola cells, which possesses arrays with the highest native curvature investigated thus far. Using cryo-ET, we reveal that Td chemoreceptor arrays assume an unusual arrangement of the supra-molecular protein assembly that has likely evolved to accommodate the high membrane curvature. The arrays have several atypical features, such as an extended dimerization domain of CheA and a variant CheW-CheR-like fusion protein that is critical for maintaining an ordered chemosensory apparatus. Furthermore, the previously characterized Td oxygen sensor ODP influences CheA ordering. These results suggest a greater diversity of the chemotaxis signaling system than previously thought.
Identifiants
pubmed: 33188180
doi: 10.1038/s41467-020-19628-6
pii: 10.1038/s41467-020-19628-6
pmc: PMC7666581
doi:
Substances chimiques
Bacterial Proteins
0
Histidine Kinase
EC 2.7.13.1
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
5763Subventions
Organisme : NIGMS NIH HHS
ID : R35 GM122535
Pays : United States
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