Analysis of Whole-Genome Sequences of Infectious laryngotracheitis Virus Isolates from Poultry Flocks in Canada: Evidence of Recombination.
herpesvirus
molecular characterization
mutation
phylogenetic analysis
poultry
recombination
vaccine
Journal
Viruses
ISSN: 1999-4915
Titre abrégé: Viruses
Pays: Switzerland
ID NLM: 101509722
Informations de publication
Date de publication:
12 11 2020
12 11 2020
Historique:
received:
03
09
2020
revised:
30
10
2020
accepted:
10
11
2020
entrez:
17
11
2020
pubmed:
18
11
2020
medline:
4
3
2021
Statut:
epublish
Résumé
Infectious laryngotracheitis virus (ILTV) is a herpes virus that causes an acute respiratory disease of poultry known as infectious laryngotracheitis (ILT). Chicken embryo origin (CEO) and tissue culture origin (TCO) live attenuated vaccines are routinely used for the control of ILT. However, vaccine virus is known to revert to virulence, and it has been recently shown that ILT field viral strains can undergo recombination with vaccinal ILTV and such recombinant ILT viruses possess greater transmission and pathogenicity potential. Based on complete or partial genes of the ILTV genome, few studies genotyped ILTV strains circulating in Canada, and so far, information is scarce on whole-genome sequencing or the presence of recombination in Canadian ILTV isolates. The objective of this study was to genetically characterize the 14 ILTV isolates that originated from three provinces in Canada (Alberta, British Columbia and Quebec). To this end, a phylogenetic analysis of 50 ILTV complete genome sequences, including 14 sequences of Canadian origin, was carried out. Additional phylogenetic analysis of the unique long, unique short and inverted repeat regions of the ILTV genome was also performed. We observed that 71%, 21% and 7% of the ILTV isolates were categorized as CEO revertant, wild-type and TCO vaccine-related, respectively. The sequences were also analyzed for potential recombination events, which included evidence in the British Columbia ILTV isolate. This event involved two ILTV vaccine (CEO) strains as parental strains. Recombination analysis also identified that one ILTV isolate from Alberta as a potential parental strain for a United States origin ILTV isolate. The positions of the possible recombination breakpoints were identified. These results indicate that the ILTV wild-type strains can recombine with vaccinal strains complicating vaccine-mediated control of ILT. Further studies on the pathogenicity of these ILTV strains, including the recombinant ILTV isolate are currently ongoing.
Identifiants
pubmed: 33198373
pii: v12111302
doi: 10.3390/v12111302
pmc: PMC7696358
pii:
doi:
Substances chimiques
DNA, Viral
0
Viral Vaccines
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Références
Vet Immunol Immunopathol. 2015 Apr 15;164(3-4):170-8
pubmed: 25764942
Bioinformatics. 2001 Aug;17(8):754-5
pubmed: 11524383
Genetics. 2007 Jun;176(2):1035-47
pubmed: 17409078
Avian Pathol. 2007 Apr;36(2):167-76
pubmed: 17479379
PLoS One. 2016 Feb 18;11(2):e0149529
pubmed: 26890525
J Virol. 2009 Apr;83(7):3127-37
pubmed: 19153224
Avian Pathol. 2006 Aug;35(4):286-92
pubmed: 16854641
Bioinformatics. 2000 Jul;16(7):573-82
pubmed: 11038328
J Mol Evol. 1992 Feb;34(2):126-9
pubmed: 1556748
Proc Natl Acad Sci U S A. 2001 Nov 20;98(24):13757-62
pubmed: 11717435
Avian Dis. 2019 Mar 14;63(2):351-358
pubmed: 31251537
World J Virol. 2012 Oct 12;1(5):142-9
pubmed: 24175219
Bioinformatics. 2000 Jun;16(6):562-3
pubmed: 10980155
PLoS One. 2018 Mar 7;13(3):e0193964
pubmed: 29513732
Mol Biol Evol. 2013 Apr;30(4):772-80
pubmed: 23329690
J Virol Methods. 2007 Jan;139(1):31-8
pubmed: 17030068
Avian Dis. 2019 Jan 28;63(2):325-334
pubmed: 31251534
Virology. 1999 Dec 20;265(2):218-25
pubmed: 10600594
Infect Genet Evol. 2020 Mar;78:104045
pubmed: 31698116
Avian Pathol. 2014;43(2):108-17
pubmed: 24460399
J Virol. 1999 Jan;73(1):152-60
pubmed: 9847317
PLoS One. 2019 Feb 7;14(2):e0211158
pubmed: 30730935
Avian Pathol. 1995 Sep;24(3):373-91
pubmed: 18645796
Aust Vet J. 2011 Mar;89(3):89-94
pubmed: 21323656
J Infect Dis. 2020 Mar 28;221(8):1271-1279
pubmed: 31016321
Avian Pathol. 1986;15(3):581-95
pubmed: 18766556
Trop Anim Health Prod. 2015 Aug;47(6):1101-8
pubmed: 25904510
Bioinformatics. 2010 Oct 1;26(19):2462-3
pubmed: 20798170
Bioinformatics. 2012 Jun 15;28(12):1647-9
pubmed: 22543367
Viruses. 2019 Jan 28;11(2):
pubmed: 30696089
PLoS One. 2015 Jul 17;10(7):e0132747
pubmed: 26186451
Animals (Basel). 2020 Sep 11;10(9):
pubmed: 32932922
PLoS One. 2013;8(2):e55121
pubmed: 23383306
Can Vet J. 1976 Apr;17(4):101-8
pubmed: 177165
J Virol. 2015 Jul;89(14):7133-46
pubmed: 25926648
Avian Dis. 1996 Jul-Sep;40(3):494-500
pubmed: 8883776
J Virol. 2007 Dec;81(23):13158-67
pubmed: 17881457
Proc Natl Acad Sci U S A. 2019 Mar 19;116(12):5693-5698
pubmed: 30819890
Appl Environ Microbiol. 2017 Nov 16;83(23):
pubmed: 28939604
Science. 2012 Jul 13;337(6091):188
pubmed: 22798607
Infect Genet Evol. 2017 Apr;49:174-185
pubmed: 28017915
Virology. 2013 May 25;440(1):64-74
pubmed: 23537957
Vaccine. 2019 Aug 14;37(35):5035-5043
pubmed: 31300291
Avian Dis. 1991 Apr-Jun;35(2):348-55
pubmed: 1649591
Vet Res. 2007 Mar-Apr;38(2):261-79
pubmed: 17296156
PLoS One. 2020 Mar 2;15(3):e0229082
pubmed: 32119681