Plant hairy roots enable high throughput identification of antimicrobials against Candidatus Liberibacter spp.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
16 11 2020
16 11 2020
Historique:
received:
10
01
2020
accepted:
26
10
2020
entrez:
17
11
2020
pubmed:
18
11
2020
medline:
26
11
2020
Statut:
epublish
Résumé
A major bottleneck in identifying therapies to control citrus greening and other devastating plant diseases caused by fastidious pathogens is our inability to culture the pathogens in defined media or axenic cultures. As such, conventional approaches for antimicrobial evaluation (genetic or chemical) rely on time-consuming, low-throughput and inherently variable whole-plant assays. Here, we report that plant hairy roots support the growth of fastidious pathogens like Candidatus Liberibacter spp., the presumptive causal agents of citrus greening, potato zebra chip and tomato vein greening diseases. Importantly, we leverage the microbial hairy roots for rapid, reproducible efficacy screening of multiple therapies. We identify six antimicrobial peptides, two plant immune regulators and eight chemicals which inhibit Candidatus Liberibacter spp. in plant tissues. The antimicrobials, either singly or in combination, can be used as near- and long-term therapies to control citrus greening, potato zebra chip and tomato vein greening diseases.
Identifiants
pubmed: 33199718
doi: 10.1038/s41467-020-19631-x
pii: 10.1038/s41467-020-19631-x
pmc: PMC7669877
doi:
Substances chimiques
Anti-Infective Agents
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
5802Références
PLoS One. 2017 Feb 3;12(2):e0171531
pubmed: 28158295
Appl Environ Microbiol. 2005 Feb;71(2):826-34
pubmed: 15691937
Front Plant Sci. 2018 Mar 02;9:268
pubmed: 29552023
Development. 2007 Sep;134(17):3099-109
pubmed: 17652352
Front Plant Sci. 2020 Mar 11;11:277
pubmed: 32218796
Nature. 2020 Oct;586(7828):311-316
pubmed: 32788727
Trends Biotechnol. 2012 Sep;30(9):475-84
pubmed: 22770837
Mol Cell Probes. 1999 Oct;13(5):373-9
pubmed: 10508559
Front Plant Sci. 2019 Jan 31;9:1890
pubmed: 30766544
Methods. 2001 Dec;25(4):402-8
pubmed: 11846609
Stand Genomic Sci. 2012 Dec 19;7(2):271-83
pubmed: 23408754
Appl Environ Microbiol. 2010 Apr;76(8):2445-50
pubmed: 20173072
Cell. 2018 May 31;173(6):1454-1467.e15
pubmed: 29656896
Mol Biol Evol. 2018 Jun 1;35(6):1547-1549
pubmed: 29722887
Plant Dis. 2013 Sep;97(9):1195-1199
pubmed: 30722426
Phytopathology. 2014 Jan;104(1):15-26
pubmed: 23883155
Science. 2015 Aug 21;349(6250):860-4
pubmed: 26184915
PLoS One. 2014 Jan 08;9(1):e84469
pubmed: 24416233
Plant Physiol. 2011 Jan;155(1):384-98
pubmed: 21030506
Plant Cell. 2007 Feb;19(2):395-402
pubmed: 17293565
Plant Physiol. 2013 Jun;162(2):755-68
pubmed: 23575417
PLoS One. 2011 Apr 28;6(4):e19135
pubmed: 21552483
Plant Physiol. 2017 Sep;175(1):23-35
pubmed: 28663331
Front Plant Sci. 2019 Mar 20;10:277
pubmed: 30949186
Ann N Y Acad Sci. 1952 Aug 8;55(2):236-47
pubmed: 12977040
Proc Soc Exp Biol Med. 1946 Nov;63(2):427-30
pubmed: 20277773
Plant Cell. 2020 Jan;32(1):166-185
pubmed: 31690653
Sci Rep. 2016 Dec 13;6:39020
pubmed: 27958354
Science. 2002 May 10;296(5570):1127-9
pubmed: 12004133
Virology. 2007 Nov 10;368(1):205-16
pubmed: 17651777
Phytopathology. 2019 Oct;109(10):1811-1819
pubmed: 31090497
Planta. 2006 May;223(6):1344-54
pubmed: 16575594
Plant Dis. 2008 Nov;92(11):1547-1550
pubmed: 30764444
Plant Methods. 2018 Feb 23;14:17
pubmed: 29483937
PLoS One. 2012;7(2):e31263
pubmed: 22347455
Genet Eng (N Y). 2002;24:67-85
pubmed: 12416301
PLoS One. 2018 Dec 28;13(12):e0209835
pubmed: 30592744
Plant Physiol. 2014 Oct;166(2):455-69
pubmed: 24868032
Plant J. 2001 Oct;28(2):123-33
pubmed: 11722756
J Eukaryot Microbiol. 2011 Sep-Oct;58(5):424-5
pubmed: 21699623
Phytopathology. 2019 Jul;109(7):1092-1101
pubmed: 30998129
FEBS Lett. 1998 Sep 18;435(2-3):159-62
pubmed: 9762899
J Eukaryot Microbiol. 2007 Nov-Dec;54(6):465-7
pubmed: 18070323
Biotechnol Adv. 2000 Mar;18(1):1-22
pubmed: 14538116
Plant Signal Behav. 2009 Aug;4(8):713-7
pubmed: 19820324
Front Plant Sci. 2015 Apr 10;6:235
pubmed: 25914712
PLoS One. 2015 Sep 23;10(9):e0137134
pubmed: 26398891
Phytopathology. 2010 Sep;100(9):949-58
pubmed: 20701493
PLoS One. 2014 Mar 28;9(3):e93475
pubmed: 24682175
Nature. 1987 Sep 17-23;329(6136):219-22
pubmed: 2442619
Front Plant Sci. 2015 Apr 08;6:131
pubmed: 25904921
Plant Physiol. 2020 Feb;182(2):882-891
pubmed: 31818905
J Infect Dis. 1957 Jul-Aug;101(1):100-8
pubmed: 13463388
Plant Dis. 2015 Aug;99(8):1125-1132
pubmed: 30695933
Microbiome. 2017 Aug 10;5(1):97
pubmed: 28797279
Plant Cell. 2000 Nov;12(11):2175-90
pubmed: 11090217
Int J Syst Evol Microbiol. 2001 Jan;51(Pt 1):89-103
pubmed: 11211278
Insects. 2019 Nov 23;10(12):
pubmed: 31771154
Phytopathology. 2019 Dec;109(12):2046-2054
pubmed: 31369360
PLoS One. 2011;6(8):e23289
pubmed: 21876745
PLoS One. 2014 Nov 05;9(11):e111032
pubmed: 25372135
Nat Commun. 2018 Nov 20;9(1):4894
pubmed: 30459421
Proc Natl Acad Sci U S A. 1995 Jul 3;92(14):6602-6
pubmed: 11607555
Phytopathology. 2016 Jan;106(1):37-46
pubmed: 26390185
Sci Rep. 2019 Mar 26;9(1):5150
pubmed: 30914689
Mol Plant. 2014 Sep;7(9):1494-1496
pubmed: 24719468
Plant Physiol. 2012 Apr;158(4):2028-41
pubmed: 22291202
Genes Dev. 2002 May 1;16(9):1139-49
pubmed: 12000796
Plant Cell. 2012 Sep;24(9):3795-804
pubmed: 22960909
Phytopathology. 2011 Nov;101(11):1285-91
pubmed: 21770778
Plant Sci. 2011 Mar;180(3):439-46
pubmed: 21421390
Macromol Biosci. 2018 Jul;18(7):e1800159
pubmed: 29900701
J Plant Physiol. 2010 Jan 15;167(2):144-8
pubmed: 19716624
Jpn J Med Sci Biol. 1967 Apr;20(2):111-25
pubmed: 4861620
Science. 1949 Jan 28;109(2822):85-7
pubmed: 17794160
Phytopathology. 2009 May;99(5):480-6
pubmed: 19351243
Plant J. 2014 Jul;79(2):348-59
pubmed: 24836556
Phytopathology. 2016 Jul;106(7):702-9
pubmed: 27050571
J Microbiol Methods. 2009 Jul;78(1):59-65
pubmed: 19409423
Trends Genet. 2013 Jul;29(7):385-93
pubmed: 23684842
Proc Natl Acad Sci U S A. 2019 Sep 3;116(36):18009-18014
pubmed: 31427509