Comparative analysis of virulence determinants, phylogroups, and antibiotic susceptibility patterns of typical versus atypical Enteroaggregative E. coli in India.
Anti-Bacterial Agents
/ therapeutic use
Bacterial Outer Membrane Proteins
/ genetics
Child
Child, Preschool
DNA, Bacterial
/ genetics
Diarrhea
/ epidemiology
Disk Diffusion Antimicrobial Tests
Escherichia coli
/ drug effects
Escherichia coli Infections
/ drug therapy
Escherichia coli Proteins
/ genetics
Feces
/ microbiology
Female
Humans
India
/ epidemiology
Infant
Male
Molecular Diagnostic Techniques
Receptors, Cell Surface
/ genetics
Trans-Activators
/ genetics
Virulence Factors
/ genetics
Journal
PLoS neglected tropical diseases
ISSN: 1935-2735
Titre abrégé: PLoS Negl Trop Dis
Pays: United States
ID NLM: 101291488
Informations de publication
Date de publication:
11 2020
11 2020
Historique:
received:
17
03
2020
accepted:
01
09
2020
entrez:
18
11
2020
pubmed:
19
11
2020
medline:
26
1
2021
Statut:
epublish
Résumé
Enteroaggregative Escherichia coli (EAEC) is an evolving enteric pathogen that causes acute and chronic diarrhea in developed and industrialized nations in children. EAEC epidemiology and the importance of atypical EAEC (aEAEC) isolation in childhood diarrhea are not well documented in the Indian setting. A comparative analysis was undertaken to evaluate virulence, phylogeny, and antibiotic sensitivity among typical tEAEC versus aEAEC. A total of 171 EAEC isolates were extracted from a broad surveillance sample of diarrheal (N = 1210) and healthy children (N = 550) across North India. Polymerase chain reaction (PCR) for the aggR gene (master regulator gene) was conducted to differentiate tEAEC and aEAEC. For 21 virulence genes, we used multiplex PCR to classify possible virulence factors among these strains. Phylogenetic classes were identified by a multiplex PCR for chuA, yjaA, and a cryptic DNA fragment, TspE4C2. Antibiotic susceptibility was conducted by the disc diffusion method as per CLSI guidelines. EAEC was associated with moderate to severe diarrhea in children. The prevalence of EAEC infection (11.4%) was higher than any other DEC group (p = 0.002). tEAEC occurrence in the diarrheal group was higher than in the control group (p = 0.0001). tEAEC strain harbored more virulence genes than aEAEC. astA, aap, and aggR genes were most frequently found in the EAEC from the diarrheal population. Within tEAEC, this gene combination was present in more than 50% of strains. Also, 75.8% of EAEC strains were multidrug-resistant (MDR). Phylogroup D (43.9%) and B1 (39.4%) were most prevalent in the diarrheal and control group, respectively. Genetic analysis revealed EAEC variability; the comparison of tEAEC and aEAEC allowed us to better understand the EAEC virulence repertoire. Further microbiological and epidemiological research is required to examine the pathogenicity of not only typical but also atypical EAEC.
Identifiants
pubmed: 33206643
doi: 10.1371/journal.pntd.0008769
pii: PNTD-D-20-00440
pmc: PMC7673547
doi:
Substances chimiques
AAP protein, E coli
0
AggR protein, E coli
0
Anti-Bacterial Agents
0
Bacterial Outer Membrane Proteins
0
ChuA protein, E coli
0
DNA, Bacterial
0
Escherichia coli Proteins
0
Receptors, Cell Surface
0
Trans-Activators
0
Virulence Factors
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0008769Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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