SARS-CoV-2 Cell Entry Factors ACE2 and TMPRSS2 Are Expressed in the Microvasculature and Ducts of Human Pancreas but Are Not Enriched in β Cells.


Journal

Cell metabolism
ISSN: 1932-7420
Titre abrégé: Cell Metab
Pays: United States
ID NLM: 101233170

Informations de publication

Date de publication:
01 12 2020
Historique:
received: 01 09 2020
revised: 19 10 2020
accepted: 10 11 2020
pubmed: 19 11 2020
medline: 18 12 2020
entrez: 18 11 2020
Statut: ppublish

Résumé

Isolated reports of new-onset diabetes in individuals with COVID-19 have led to the hypothesis that SARS-CoV-2 is directly cytotoxic to pancreatic islet β cells. This would require binding and entry of SARS-CoV-2 into β cells via co-expression of its canonical cell entry factors, angiotensin-converting enzyme 2 (ACE2) and transmembrane serine protease 2 (TMPRSS2); however, their expression in human pancreas has not been clearly defined. We analyzed six transcriptional datasets of primary human islet cells and found that ACE2 and TMPRSS2 were not co-expressed in single β cells. In pancreatic sections, ACE2 and TMPRSS2 protein was not detected in β cells from donors with and without diabetes. Instead, ACE2 protein was expressed in islet and exocrine tissue microvasculature and in a subset of pancreatic ducts, whereas TMPRSS2 protein was restricted to ductal cells. These findings reduce the likelihood that SARS-CoV-2 directly infects β cells in vivo through ACE2 and TMPRSS2.

Identifiants

pubmed: 33207245
pii: S1550-4131(20)30601-X
doi: 10.1016/j.cmet.2020.11.006
pmc: PMC7664344
pii:
doi:

Substances chimiques

RNA, Messenger 0
ACE2 protein, human EC 3.4.17.23
Angiotensin-Converting Enzyme 2 EC 3.4.17.23
Serine Endopeptidases EC 3.4.21.-
TMPRSS2 protein, human EC 3.4.21.-

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Langues

eng

Sous-ensembles de citation

IM

Pagination

1028-1040.e4

Subventions

Organisme : NIDDK NIH HHS
ID : UC4 DK104211
Pays : United States
Organisme : NIDDK NIH HHS
ID : UC4 DK112232
Pays : United States
Organisme : NIDDK NIH HHS
ID : U01 DK089572
Pays : United States
Organisme : NIDDK NIH HHS
ID : U01 DK123594
Pays : United States
Organisme : NIDDK NIH HHS
ID : U01 DK123743
Pays : United States
Organisme : NIDDK NIH HHS
ID : R24 DK106755
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK117147
Pays : United States
Organisme : NIDDK NIH HHS
ID : U01 DK123716
Pays : United States
Organisme : NIDDK NIH HHS
ID : UC4 DK098085
Pays : United States
Organisme : NIDDK NIH HHS
ID : UC4 DK108120
Pays : United States
Organisme : NIDDK NIH HHS
ID : U2C DK059637
Pays : United States
Organisme : NIDDK NIH HHS
ID : U24 DK098085
Pays : United States
Organisme : Doris Duke Charitable Foundation
ID : 2020063
Pays : United States
Organisme : NHLBI NIH HHS
ID : P01 HL075462
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA068485
Pays : United States

Commentaires et corrections

Type : UpdateOf

Informations de copyright

Copyright © 2020 Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of Interests The authors declare no competing interests.

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Auteurs

Katie C Coate (KC)

Division of Diabetes, Endocrinology and Metabolism, Department of Medicine, Vanderbilt University Medical Center, Nashville, TN 37232, USA.

Jeeyeon Cha (J)

Division of Diabetes, Endocrinology and Metabolism, Department of Medicine, Vanderbilt University Medical Center, Nashville, TN 37232, USA.

Shristi Shrestha (S)

Division of Diabetes, Endocrinology and Metabolism, Department of Medicine, Vanderbilt University Medical Center, Nashville, TN 37232, USA.

Wenliang Wang (W)

Department of Genetics and Institute for Diabetes, Obesity, and Metabolism, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA 19104, USA.

Luciana Mateus Gonçalves (LM)

Division of Endocrinology, Diabetes and Metabolism, Department of Medicine, University of Miami Miller School of Medicine, Miami, FL 33136, USA.

Joana Almaça (J)

Division of Endocrinology, Diabetes and Metabolism, Department of Medicine, University of Miami Miller School of Medicine, Miami, FL 33136, USA.

Meghan E Kapp (ME)

Department of Pathology, Microbiology and Immunology, Vanderbilt University Medical Center, Nashville, TN 37232, USA.

Maria Fasolino (M)

Department of Genetics and Institute for Diabetes, Obesity, and Metabolism, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA 19104, USA.

Ashleigh Morgan (A)

Department of Genetics and Institute for Diabetes, Obesity, and Metabolism, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA 19104, USA.

Chunhua Dai (C)

Division of Diabetes, Endocrinology and Metabolism, Department of Medicine, Vanderbilt University Medical Center, Nashville, TN 37232, USA.

Diane C Saunders (DC)

Division of Diabetes, Endocrinology and Metabolism, Department of Medicine, Vanderbilt University Medical Center, Nashville, TN 37232, USA.

Rita Bottino (R)

Institute of Cellular Therapeutics, Allegheny Health Network, Pittsburgh, PA 15212, USA; Imagine Pharma, Devon, PA 19333, USA.

Radhika Aramandla (R)

Division of Diabetes, Endocrinology and Metabolism, Department of Medicine, Vanderbilt University Medical Center, Nashville, TN 37232, USA.

Regina Jenkins (R)

Division of Diabetes, Endocrinology and Metabolism, Department of Medicine, Vanderbilt University Medical Center, Nashville, TN 37232, USA.

Roland Stein (R)

Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, TN 37232, USA.

Klaus H Kaestner (KH)

Department of Genetics and Institute for Diabetes, Obesity, and Metabolism, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA 19104, USA.

Golnaz Vahedi (G)

Department of Genetics and Institute for Diabetes, Obesity, and Metabolism, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA 19104, USA.
The Human Pancreas Analysis Program.

Marcela Brissova (M)

Division of Diabetes, Endocrinology and Metabolism, Department of Medicine, Vanderbilt University Medical Center, Nashville, TN 37232, USA. Electronic address: marcela.brissova@vumc.org.

Alvin C Powers (AC)

Division of Diabetes, Endocrinology and Metabolism, Department of Medicine, Vanderbilt University Medical Center, Nashville, TN 37232, USA; VA Tennessee Valley Healthcare System, Nashville, TN 37212, USA; Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, TN 37232, USA. Electronic address: al.powers@vumc.org.

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