Assessment of
16S rRNA
PCR
amplicon
human microbiome
metagenomics
molecular methods
quantitative methods
vaginal microbiome
Journal
mSphere
ISSN: 2379-5042
Titre abrégé: mSphere
Pays: United States
ID NLM: 101674533
Informations de publication
Date de publication:
18 11 2020
18 11 2020
Historique:
entrez:
19
11
2020
pubmed:
20
11
2020
medline:
8
9
2021
Statut:
epublish
Résumé
The vaginal microbiome has been connected to a wide range of health outcomes. This has led to a thriving research environment but also to the use of conflicting methodologies to study its microbial composition. Here, we systematically assessed best practices for the sequencing-based characterization of the human vaginal microbiome. As far as 16S rRNA gene sequencing is concerned, the V1-V3 region performed best
Identifiants
pubmed: 33208514
pii: 5/6/e00448-20
doi: 10.1128/mSphere.00448-20
pmc: PMC7677004
pii:
doi:
Substances chimiques
RNA, Ribosomal, 16S
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Commentaires et corrections
Type : ErratumIn
Informations de copyright
Copyright © 2020 Hugerth et al.
Références
Gynecol Endocrinol. 2018 Jun;34(6):451-455
pubmed: 29271266
J Basic Microbiol. 2018 Nov;58(11):987-997
pubmed: 30091475
Front Med (Lausanne). 2018 Jun 13;5:181
pubmed: 29951482
J Clin Microbiol. 1991 Feb;29(2):297-301
pubmed: 1706728
Appl Environ Microbiol. 2014 Aug;80(16):5116-23
pubmed: 24928874
Proc Natl Acad Sci U S A. 2011 Mar 15;108 Suppl 1:4680-7
pubmed: 20534435
Nat Methods. 2012 Mar 04;9(4):357-9
pubmed: 22388286
Nat Methods. 2015 Oct;12(10):902-3
pubmed: 26418763
J Basic Microbiol. 2011 Aug;51(4):442-6
pubmed: 21298692
Genome Biol. 2019 Nov 28;20(1):257
pubmed: 31779668
BMC Bioinformatics. 2019 Mar 25;20(1):153
pubmed: 30909888
J Infect Dis. 2014 Dec 1;210(11):1723-33
pubmed: 24943724
Nucleic Acids Res. 2013 Jan 7;41(1):e1
pubmed: 22933715
Appl Environ Microbiol. 2013 Sep;79(17):5112-20
pubmed: 23793624
Nat Methods. 2017 Nov;14(11):1063-1071
pubmed: 28967888
Nat Commun. 2020 Feb 26;11(1):940
pubmed: 32103005
Infect Dis Obstet Gynecol. 2003;11(1):11-7
pubmed: 12839628
Nat Methods. 2016 Jul;13(7):581-3
pubmed: 27214047
Sci Rep. 2014 May 30;4:5136
pubmed: 24875844
PLoS One. 2020 Jan 16;15(1):e0227434
pubmed: 31945086
Genome Biol. 2020 Sep 10;21(1):242
pubmed: 32912225
Nat Biotechnol. 2018 Nov;36(10):996-1004
pubmed: 30148503
PLoS One. 2012;7(6):e39315
pubmed: 22720093
J Clin Microbiol. 2010 May;48(5):1812-9
pubmed: 20305015
Proc Natl Acad Sci U S A. 2011 Mar 15;108 Suppl 1:4516-22
pubmed: 20534432
Nucleic Acids Res. 2013 Jan;41(Database issue):D590-6
pubmed: 23193283
Appl Environ Microbiol. 2008 Apr;74(8):2537-9
pubmed: 18296534
Microbiologyopen. 2019 Mar;8(3):e00661
pubmed: 29931836
Clin Infect Dis. 2003 Aug 1;37(3):319-25
pubmed: 12884154
Nat Commun. 2017 Oct 17;8(1):875
pubmed: 29042534
Leg Med (Tokyo). 2012 May;14(3):160-2
pubmed: 22316585
BJOG. 2020 Jan;127(2):171-180
pubmed: 31237400
Curr HIV/AIDS Rep. 2018 Feb;15(1):30-38
pubmed: 29516267
PeerJ. 2016 Oct 18;4:e2584
pubmed: 27781170
Nucleic Acids Res. 2014 Jan;42(Database issue):D633-42
pubmed: 24288368
Curr Infect Dis Rep. 2019 Aug 1;21(9):30
pubmed: 31367983
Nat Rev Urol. 2020 Apr;17(4):232-250
pubmed: 32071434
Sci Rep. 2018 Aug 13;8(1):12034
pubmed: 30104688
BMC Microbiol. 2005 May 18;5:28
pubmed: 15904497
J Biomed Inform. 2019 Jul;95:103208
pubmed: 31078660
Res Microbiol. 2015 Jun;166(5):440-447
pubmed: 25869222
Res Microbiol. 2017 Nov - Dec;168(9-10):845-858
pubmed: 28502874
Nat Commun. 2019 Mar 21;10(1):1305
pubmed: 30899005
Gigascience. 2020 Feb 1;9(2):
pubmed: 32052832
Appl Environ Microbiol. 2008 Apr;74(8):2461-70
pubmed: 18296538
Am J Med. 1983 Jan;74(1):14-22
pubmed: 6600371
Microbiome. 2018 Jun 28;6(1):117
pubmed: 29954448
Microbiome. 2013 Dec 02;1(1):29
pubmed: 24451163