Growth and Oleanolic Acid Accumulation of Polyscias fruticosa Cell Suspension Cultures.
Cell suspension culture
Polyscias fruticosa
cell culture.
oleanane triterpene
oleanolic acid
saponin
Journal
Current pharmaceutical biotechnology
ISSN: 1873-4316
Titre abrégé: Curr Pharm Biotechnol
Pays: Netherlands
ID NLM: 100960530
Informations de publication
Date de publication:
2021
2021
Historique:
received:
29
01
2020
revised:
30
03
2020
accepted:
27
10
2020
pubmed:
21
11
2020
medline:
20
8
2021
entrez:
20
11
2020
Statut:
ppublish
Résumé
Oleanolic acid is an oleanane triterpene found in many plant species all over the world. This compound is also a major saponin in leaves of Polyscias fruticosa and possesses several promising pharmacological activities, such as hepatoprotective effects, and antiinflammatory, antioxidant, or anticancer activities. The objective of the present work is to establish cell suspension culture of P. fruticosa, investigate the influence of several factors such as plant growth regulators and carbon source on cell growth, and determine their oleanolic acid content. Cell culture was established by using 2 g fresh weight of 30 day old friable callus derived from in vitro stem segment in 50 mL of liquid medium with a shaking speed of 220 rpm. The culture was then incubated at 25±2ºC with a shaking speed of 120 rpm in the period of 12 h daylight at a light intensity of about 6.75 μmol/m2/s. Cell growth was measured by fresh and dry biomass at 16 h day. Oleanolic acid content was determined using HPLC analysis. The study results showed that MS medium containing 2% sucrose as a carbon source, supplemented with 1 mg/L 6-benzylaminopurine and 0.5 mg/L 2,4-dichlorophenoxyacetic acid was the most appropriate growth medium. Cell biomass and oleanolic acid content reached the highest values of 0.43 g dry weight/flask and 25.4 mg/g dry weight, respectively. These results indicated the potential production of oleanolic acid, a compound with high pharmacological value, from P. fruticosa cell culture.
Sections du résumé
BACKGROUND
BACKGROUND
Oleanolic acid is an oleanane triterpene found in many plant species all over the world. This compound is also a major saponin in leaves of Polyscias fruticosa and possesses several promising pharmacological activities, such as hepatoprotective effects, and antiinflammatory, antioxidant, or anticancer activities.
OBJECTIVE
OBJECTIVE
The objective of the present work is to establish cell suspension culture of P. fruticosa, investigate the influence of several factors such as plant growth regulators and carbon source on cell growth, and determine their oleanolic acid content.
METHODS
METHODS
Cell culture was established by using 2 g fresh weight of 30 day old friable callus derived from in vitro stem segment in 50 mL of liquid medium with a shaking speed of 220 rpm. The culture was then incubated at 25±2ºC with a shaking speed of 120 rpm in the period of 12 h daylight at a light intensity of about 6.75 μmol/m2/s. Cell growth was measured by fresh and dry biomass at 16 h day. Oleanolic acid content was determined using HPLC analysis.
RESULTS & DISCUSSION
CONCLUSIONS
The study results showed that MS medium containing 2% sucrose as a carbon source, supplemented with 1 mg/L 6-benzylaminopurine and 0.5 mg/L 2,4-dichlorophenoxyacetic acid was the most appropriate growth medium. Cell biomass and oleanolic acid content reached the highest values of 0.43 g dry weight/flask and 25.4 mg/g dry weight, respectively.
CONCLUSION
CONCLUSIONS
These results indicated the potential production of oleanolic acid, a compound with high pharmacological value, from P. fruticosa cell culture.
Identifiants
pubmed: 33213337
pii: CPB-EPUB-111654
doi: 10.2174/1389201021666201119123503
doi:
Substances chimiques
Saponins
0
Triterpenes
0
Oleanolic Acid
6SMK8R7TGJ
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1266-1272Informations de copyright
Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.