Crystal Structure of a Bivalent Antibody Fab Fragment.

Amino Acid Sequence Amino Acid Substitution Antibodies, Bispecific / chemistry Antibody Affinity Cryoelectron Microscopy Crystallography, X-Ray Immunoglobulin Fab Fragments / chemistry Models, Molecular Mutation Protein Conformation Protein Multimerization Recombinant Proteins Spectrum Analysis Structure-Activity Relationship Thermodynamics
antibody domain swapping fab structure

Journal

Journal of molecular biology
ISSN: 1089-8638
Titre abrégé: J Mol Biol
Pays: Netherlands
ID NLM: 2985088R

Informations de publication

Date de publication:
22 01 2021
Historique:
received: 19 08 2020
revised: 29 10 2020
accepted: 11 11 2020
pubmed: 22 11 2020
medline: 27 4 2021
entrez: 21 11 2020
Statut: ppublish

Résumé

We determined the crystal structure to 1.8 Å resolution of the Fab fragment of an affinity-matured human monoclonal antibody (HC84.26.5D) that recognizes the E2 envelope glycoprotein of hepatitis C virus (HCV). Unlike conventional Fabs, which are monovalent monomers, Fab HC84.26.5D assembles into a bivalent domain-swapped dimer in which the two V

Identifiants

DOI: 10.1016/j.jmb.2020.11.013 PMID: 33220264
pubmed: 33220264
pii: S0022-2836(20)30632-X
doi: 10.1016/j.jmb.2020.11.013
pii:
doi:

Substances chimiques

Antibodies, Bispecific 0
Immunoglobulin Fab Fragments 0
Recombinant Proteins 0

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S.

Langues

eng

Sous-ensembles de citation

IM

Pagination

166714

Informations de copyright

Copyright © 2020 Elsevier Ltd. All rights reserved.

Auteurs

Salman Shahid (S)

University of Maryland Institute for Bioscience and Biotechnology Research, Rockville, MD 20850, USA; Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD 20742, USA.

Mingming Gao (M)

University of Maryland Institute for Bioscience and Biotechnology Research, Rockville, MD 20850, USA; Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD 20742, USA.

D Travis Gallagher (D)

University of Maryland Institute for Bioscience and Biotechnology Research, Rockville, MD 20850, USA; National Institute of Standards and Technology, Gaithersburg, MD 20899, USA.

Edwin Pozharski (E)

University of Maryland Institute for Bioscience and Biotechnology Research, Rockville, MD 20850, USA; Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD 21201, USA.

Robert G Brinson (RG)

University of Maryland Institute for Bioscience and Biotechnology Research, Rockville, MD 20850, USA; National Institute of Standards and Technology, Gaithersburg, MD 20899, USA.

Zhen-Yong Keck (ZY)

Department of Pathology, Stanford University School of Medicine, Stanford, CA 94304, USA.

Steven K H Foung (SKH)

Department of Pathology, Stanford University School of Medicine, Stanford, CA 94304, USA.

Thomas R Fuerst (TR)

University of Maryland Institute for Bioscience and Biotechnology Research, Rockville, MD 20850, USA; Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD 20742, USA.

Roy A Mariuzza (RA)

University of Maryland Institute for Bioscience and Biotechnology Research, Rockville, MD 20850, USA; Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD 20742, USA. Electronic address: rmariuzz@umd.edu.

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Classifications MeSH

questionsmedicales.fr › Sciences de l'information › Sources d'information › Services d'information › Documentation › Données de séquences moléculaires › Séquence d'acides aminés › Crystal Structure of a Bivalent Antibody Fab Fragment.
questionsmedicales.fr › Phénomènes génétiques › Mutagenèse › Substitution d'acide aminé › Crystal Structure of a Bivalent Antibody Fab Fragment.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Globulines › Sérum-globulines › Immunoglobulines › Anticorps › Anticorps bispécifiques › Crystal Structure of a Bivalent Antibody Fab Fragment.
questionsmedicales.fr › Phénomènes du système immunitaire › Réaction antigène-anticorps › Affinité des anticorps › Crystal Structure of a Bivalent Antibody Fab Fragment.
questionsmedicales.fr › Techniques d'investigation › Microscopie électronique › Cryomicroscopie électronique › Crystal Structure of a Bivalent Antibody Fab Fragment.
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Cristallographie aux rayons X › Crystal Structure of a Bivalent Antibody Fab Fragment.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Globulines › Sérum-globulines › Immunoglobulines › Fragments d'immunoglobuline › Fragments Fab d'immunoglobuline › Crystal Structure of a Bivalent Antibody Fab Fragment.
questionsmedicales.fr › Techniques d'investigation › Modèles théoriques › Modèles moléculaires › Crystal Structure of a Bivalent Antibody Fab Fragment.
questionsmedicales.fr › Phénomènes génétiques › Variation génétique › Mutation › Crystal Structure of a Bivalent Antibody Fab Fragment.
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Conformation moléculaire › Conformation des protéines › Crystal Structure of a Bivalent Antibody Fab Fragment.
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Multimérisation de protéines › Crystal Structure of a Bivalent Antibody Fab Fragment.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines recombinantes › Crystal Structure of a Bivalent Antibody Fab Fragment.
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Analyse spectrale › Crystal Structure of a Bivalent Antibody Fab Fragment.
questionsmedicales.fr › Phénomènes physiologiques › Phénomènes pharmacologiques et toxicologiques › Phénomènes pharmacologiques › Relation structure-activité › Crystal Structure of a Bivalent Antibody Fab Fragment.
questionsmedicales.fr › Phénomènes physiques › Thermodynamique › Crystal Structure of a Bivalent Antibody Fab Fragment.